Abstract: In the present paper, 14 characters were measured and analyzed by means of principle component analysis method to 131 specimens belonging to the genus Pseudogobio. The results showed that the diagnostic characters between P. guilinensis and P. vaillanti, such as end of pectoral fin over or not reach ventral origin, ratio between length and depth of caudal peduncle, are correlated. However, specimens can not be distinguished definitely according to these differences because of the overlap in characters. There are differences among populations from different localities but no correlations to north-south or east-west geographical differentiations can be found. Based on these results, Pseudogobio species in China was being suggested as only one species and P. guilinensis as a synonym of P. vaillanti.