盐酸环丙沙星在中华绒螯蟹体内药物代谢动力学研究
PHARMACOKINETICS OF CIPROFLOXACINUM IN CHINESE MITTEN-HANDED CRAB, ERIOCHEIR SINENSIS
-
摘要: 应用反向高效液相色谱法对盐酸环丙沙星在中华绒螯蟹雄、雌蟹血淋巴与肌肉内的代谢规律进行了研究.肌肉注射给药后血淋巴中盐酸环丙沙星的浓度立即达到峰值,药物开始向肌肉等组织中运转,血药浓度在1h内迅速下降,而肌肉中的浓度达到峰值;此后无论是血淋巴还是肌肉中的盐酸环丙沙星浓度均缓慢下降,到第120h雌、雄蟹中盐酸环丙沙星的浓度均在1μg·mL-1(g-1)以下,到216h均不能检出.研究表明:中华绒螯蟹血淋巴中盐酸环丙沙星的药物浓度可用一级吸收二室开放模型描述,雄、雌蟹的消除半衰期(T1/2β)分别为40.34±1.48h和22.07±0.31h;中华绒螯蟹的性别对盐酸环丙沙星在其体内的代谢有较大的影响.Abstract: To study the metabolic law of Ciprofloxacinum in Chinese mitten-handed Crab,Eriocheir sinensis,its concentrations both in hemolymph and muscle were measured by using RP-HPLC after intramuscular injection at a dose of 8.17±0.56mg·kg-1and 6.25±0.85mg·kg-1 to male and female crab at the first experiment,or at a dose of 5.99±0.27mg·kg-1 and 6 05±0 32mg·kg-1at the second experiment respectively The concentration of Ciprofloxacinum in the hemolymph was the highest immediately after injection Within an hour,the blood-drug concentration dropped sharply On the other hand,the concentration in the muscle reached maximum an hour after injection and decreased gradually thereafter It was lower than 1μg·mL-1(g-1)at 120h and undetectable at 216h This finding indicated that the injected Ciprofloxacinum moved into muscles via hemolymph in the crab.The concentrations of Ciprofloxacinum in hemolymph were best described by a two-compartment open model with first-order absorption To male and female crab,the pharmacokinetic parameter,T 1/2β,was 40.34±1.48h and 22.07±0.31h respectively Also,Pharmacokinetic difference between male and female crab was observed.
-
Keywords:
- Eriocheir sinensis /
- Ciprofloxacinum /
- Muscle /
- Pharmacokinetics
-
-
[1] Yang J. Fisheries medicine manual[M]. Beijing: Science and Technology Press of China. 1998, 179—180.[杨坚主编,渔药手册.北京:中国科学技术出版社.1998,179-180][2] Li T W, Ding M J, Song X M, et al. Preliminary studies on the mechanism of Vibrio fluvialis-Ⅱ resistance to antibiotics. Oceanologia ET Limnoligia Sinica,1996,27(6):637—645[李太武,丁明进,宋协民,等.皱纹盘鲍脓胞病病原菌--河流弧菌-Ⅱ的抗药机制的初步研究.海洋与湖沼,1996,27(6):637-645][3] Lin T R, Yu N F. The synthetical treatment for Trionyx sinensis with coexistence symptom of acute gastroenteritis and branchial inflammation[J]. Aquaculture of interior Land. 1997, 7: 25.[林天然,余乃凤.甲鱼急性肠胃炎鳃腺炎并发症的综合治疗.内陆水产,1997,7:25][4] Meng Q X, Yu K K. The diagnosis, prevention and cure for the disease of fish, shrimp, crab and shellfish[M]. Bingjing: Agriculture Press of China. 1996,255—281.[孟庆显,余开康.鱼虾蟹贝疾病诊治和防治.北京:中国农业出版社.1996,255-281][5] Yang X L, Liu Z Z, Sun W Q, et al. The method of RP-HPLC of ciprofloxacinum in hemolymph of Eriocheir sinenses[J]. Journal of Fisheries of China. 2000,25(4): 348-355.[杨先乐,刘至治,孙文钦,等.中华绒螯蟹血淋巴内盐酸环丙沙星的反相高效液相色谱法的建立.水产学报,2001,25(4):348-355][6] Cai W C, Li B Y, Li Y M. The experimental technique tutorial of Bio-chemical[M]. Shanghai: Fudan University Press. 1983: 47. [蔡武城,李碧羽,李玉民.生化实验技术教程[M].上海:复旦大学出版社.1983:47] [7] Nouws J F M,Grondel J L,Schutte A R,et al., Pharmacokinetics of ciprofloxacin in carp, African catfish and rainbow.trout[J].Vet-Quart,1988,10:2—216[8] Zhang Z X, Zhang Y B, Yang R S. The pharmacokinetic of Norfloxacin studies on common crap[J]. Journal of veterinary of China. 2000, 20(1): 66-69.[张作新,张雅斌,杨永胜.诺氟沙星在鲤鱼体内的药代动力学.中国兽医学报,2000,20(1):66-69] Yang J. Fisheries medicine manual[M]. Beijing: Science and Technology Press of China. 1998, 179—180.[杨坚主编,渔药手册.北京:中国科学技术出版社.1998,179-180][2] Li T W, Ding M J, Song X M, et al. Preliminary studies on the mechanism of Vibrio fluvialis-Ⅱ resistance to antibiotics. Oceanologia ET Limnoligia Sinica,1996,27(6):637—645[李太武,丁明进,宋协民,等.皱纹盘鲍脓胞病病原菌--河流弧菌-Ⅱ的抗药机制的初步研究.海洋与湖沼,1996,27(6):637-645][3] Lin T R, Yu N F. The synthetical treatment for Trionyx sinensis with coexistence symptom of acute gastroenteritis and branchial inflammation[J]. Aquaculture of interior Land. 1997, 7: 25.[林天然,余乃凤.甲鱼急性肠胃炎鳃腺炎并发症的综合治疗.内陆水产,1997,7:25][4] Meng Q X, Yu K K. The diagnosis, prevention and cure for the disease of fish, shrimp, crab and shellfish[M]. Bingjing: Agriculture Press of China. 1996,255—281.[孟庆显,余开康.鱼虾蟹贝疾病诊治和防治.北京:中国农业出版社.1996,255-281][5] Yang X L, Liu Z Z, Sun W Q, et al. The method of RP-HPLC of ciprofloxacinum in hemolymph of Eriocheir sinenses[J]. Journal of Fisheries of China. 2000,25(4): 348-355.[杨先乐,刘至治,孙文钦,等.中华绒螯蟹血淋巴内盐酸环丙沙星的反相高效液相色谱法的建立.水产学报,2001,25(4):348-355][6] Cai W C, Li B Y, Li Y M. The experimental technique tutorial of Bio-chemical[M]. Shanghai: Fudan University Press. 1983: 47. [蔡武城,李碧羽,李玉民.生化实验技术教程[M].上海:复旦大学出版社.1983:47] [7] Nouws J F M,Grondel J L,Schutte A R,et al., Pharmacokinetics of ciprofloxacin in carp, African catfish and rainbow.trout[J].Vet-Quart,1988,10:2—216[8] Zhang Z X, Zhang Y B, Yang R S. The pharmacokinetic of Norfloxacin studies on common crap[J]. Journal of veterinary of China. 2000, 20(1): 66-69.[张作新,张雅斌,杨永胜.诺氟沙星在鲤鱼体内的药代动力学.中国兽医学报,2000,20(1):66-69]
计量
- 文章访问数: 1019
- HTML全文浏览量: 1
- PDF下载量: 479
下载: