草鱼和鲤群体遗传变异的RAPD指纹分析

ASSESSMENT OF POPULATION GENETIC VARIATION OF GRASS CARP AND COMMON CARP USING RAPD FINGER PRINTS

  • 摘要: 利用随机扩增多态DNA(RAPD)技术对草鱼、兴国红鲤、野鲤的种群内、种群间以及种间的遗传变异进行了定量分析。结果表明:草鱼与鲤的RAPD指纹图谱带型差异显著,草鱼与红鲤和鲤种间的平均带纹相似系数(ABS)分别为0.2583和0.2394,遗传距离(Dij)分别达0.9362和1.2277。而红鲤和鲤种群间的RAPD指纹图谱带型则基本一致,平均带纹相似系数为0.7162,遗传距离只有0.0947。草鱼、兴国红鲤、鲤种群内平均带纹相似系数分别为0.8424、0.7819,0.7928,平均带纹频率(F)分别为0.82、0.71和0.74,相应的遗传变异度分别为0.18、0.29和0.26。

     

    Abstract: Intra-and inter-population genetic variation of grass carp, red common carp and common carp was analysed using random amplified polymorphic DNA (RAPD) markers. The RAPD patterns of grass carp (Ctenophoryngodon idellus) were remarkably different from those of common carp (Cyprinus carpio), and the inter-population average band sharing between grass carp and the two common carps (red common carp and common carp) were 0.2583 and 0.2394, respectively, and the cormsponding genetic distances between them were 0.9362 and 1.2277. However, the RAPD patterns of red common carp and common carp were very similar, with the average band sharing between them being 0.7162 and genetic distance 0.0947. The intra-population average band sharing of grass carp, red common carp and common carp were 0.8424, 0.7819 and 0.7928, respectively, average band frequency were 0.82,0.71 and 0.74, respectively, and the genetic variability were 0.18, 0.29 and 0.26,respectively.

     

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