大田软海绵酸对FL细胞DNA的损伤及凋亡相关蛋白表达的影响
DNA DAMAGE AND ALTERATION OF EXPRESSION IN APOPTOSIS RELATED PROTEINS INDUCED BY OKADAICACID IN FL CELLS
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摘要: 本文旨在探讨大田软海绵酸对人羊膜细胞DNA的损伤及凋亡相关蛋白表达的影响。实验用0、20、40、608、0、100nmol/LOA诱导FL细胞4h后,检测DNA损伤程度的彗星实验表明,OA对FL细胞DNA的损伤随染毒浓度的升高而增加。蛋白免疫印迹法显示凋亡相关蛋白Bcl-2、Bax和p53的表达与染毒浓度呈负相关;用100nmol/LOA分别诱导2h、4h、8h后发现,三种蛋白的表达与染毒时间也呈负相关。由此可知在OA诱导的FL细胞凋亡中,损伤DNA,降低Bcl-2蛋白的表达可能参与了凋亡的部分作用,而Bax和p53蛋白则可能与OA诱导的细胞增殖有关。Abstract: The objective of this work was to evaluate deoxyribonucleic acid (DNA) damage and expression of Bcl-2, Bax, p53 proteins in okadaic acid treated human amniotic FL cells1 Cells were cultured for 4 h with 0, 20, 40, 60, 80 and 100 nmol okadaic acid (OA), or 100 nmol OA for periods of 2, 4 and 8 h1 Comet assay results indicated that DNA damage was a dose-dependent increase and western blot analysis showed that the expression of Bcl 2, Bax, p53 proteins was time and dose dependent decrease1 Therefore, it suggested that DNA damage and Bcl-2 might play a partial role in OA-induced FL cell apoptosis, and on the contrary, Bax and p53 were related to the proliferation of FL cell1
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Keywords:
- Okadaic acid /
- DNA damage /
- Bc- l 2 /
- Bax /
- p53
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[1] Fern àndez J J, Candenas M L, Souto M L, et al. Okadaic acid,useful tool for studying cellular processes [J]. Curr Med Chem,2002,9: 229-262
[2] Lago J, Santaclara F, Vieites J M, et al. Collapse of mitochondrial membrane potential and caspases activation are early events in okadaic acidtreated Cacocell [J]. Toxicon, 2005, 46(5): 579-586
[3] Fujiki H, Sueoka E, Komori A, et al. Tumor promotion and TNFagene expression by the okadaic acid class tumor promoters [J]. Environ Carcinog Ecotoxicol Rev, 1997, 15: 1-40
[4] Matias W G, Creppy E E. Transplacental passage of [3H]-okadaic acidin pregnant mice measured by radioactivity and high-performance liquid chromat ography [J]. HumExp Toxicol, 1996, 15(3): 226-230
[5] Singh N P, McCoy M T, Tice R R, et al. A simple technique forquantitation of low levels of DNA damage in individual cells [J].Exp Cell Res, 1988, 175(1): 184-191
[6] Moncada A, Cendan C M, Baeyens J M, et al. Effects of serine/threonine protein phosphatase inhibitors on morphine induced antinociception in the tail flick test in mice [J]. Eur J Pharmacol,2003, 465: 53-60
[7] Le Hegarat L, Puech L, Fessard V, et al. Aneugenic potential ofokadaic acid revealed by the micronucleus assay combined with theFISH technique in CHOK1 cells [J]. Mutagenesis, 2003, 18(3):293-298
[8] Xing M L, Chen J P, Wang X F, et al. Effect of okadaic acid onapoptosis in human amnion cells [J]. Acta Hydrobiologica Sinica,2007, 31(4): 606-608 [邢鸣鸾, 陈加平, 王晓峰, 等. 大田软海绵酸对 FL 细胞凋亡的影响. 水生生物学报, 2007, 31(4):606-608]
[9] Traore A, Baudrimont I.Ambaliou S, et al. DNA breaks and cell cycle arrest induced by okadaic acid in Cacocells, a human colonicepithelial cell line [J]. Arch Toxico, 2001,75(2): 110-117
[10] Bandyopadhyay S, Sengupta T K, Fernandes D J, et al. Taxol andokadaic acidinduced destabilization of bcl 2mRNA is associated withdecreased binding of proteins to a bcl 2 instability element [J].Biochem Pharmacol, 2003,66(7): 1151-1162
[11] Milczarek G J, Chen W, Gupta A, et al. Okadaic acid mediates p53hyperphosphorylation and growth arrest in cells with wild type p53 butincreases aberrant mitoses in cells with nonfunctional p53 [J]. Carcinogenesis, 1999, 20(6): 1043-1048
[12] Yoon S Y, Choi J E, Yoon J, et al. Okadaic acid induces JNK activation, bim overexpression and mitochondrial dysfunction in culturedrat cortical neurons [J]. Neurosci Lett, 2006, 394(3): 190-195
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