生物异源物质对小球藻基因转录研究体系的建立(英文)

基金项目:

National nature Science Foundation of “Enantioselectivity of toxicities and molecular mechanisms in chiral herbicides”

Natural Science Foundation of China(20607020)

Ministry of Education Key Lab of Environment Remediation and Ecological Health of China(EREH0709)

ESTABLISHMENT OF REAL-TIME PCR FOR ANALYZING mRNA ABUNDANCE IN CHLORELLA VULGARIS EXPOSED TO XENOBIOTICS

College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032 China;
2. Key Lab of Environment Remediation and Ecological Health, College of Environmental and Resource, Zhejiang University, Hangzhou 310029 China

摘要: 藻类是水生生态系统中的敏感物种。在环境生物学研究中,基因转录水平通常作为衡量基因表达水平变化的指标。研究建立了定量PCR技术(Real-time PCR)检测小球藻基因转录变化的研究体系。以3个主要光合作用基因(psaB、psbC、rbcL)为靶标基因,研究了除草剂草丁瞵(Glufosinate)、阿特拉津(Atrazine)和禾草灵(Diclofop-methyl)对基因转录的影响,结果表明3种供试药物对小球藻光合作用基因psbC和rbcL相对表达量有显著地抑制作用。这表明Real-time PCR可以作为评定水生环境中异源化合物毒性的有效方法。
- Real-timePCR /
- 普通小球藻 /
- 光合作用 /
- 基因转录
Abstract: Among the aquatic organisms,algae are one of the most susceptible organisms to pollution in water.Transcriptional abundance has been gradually used as a surrogate for gene expression in environmental biology.In the present study,real-time PCR assay was established to quantify the transcript abundances of three photosystem genes(psaB,psbC and rbcL) in Chlorella vulgaris,and the acute toxicity of three herbicides(glufosinate,atrazine and diclofop-methyl) was assessed using this method.The results demonstrated that real-time PCR is an efficient technique to assess the toxicity of xenobiotic compounds in aquatic system.
- Real-time PCR /
- Chlorella vulgaris /
- Photosynthesis /
- Transcript abundance