二温式多重PCR检测对虾白斑综合征病毒和桃拉病毒的研究

基金项目:

广西科技攻关项目资助(桂科攻0322006-3A)

STUDY ON THE DETECTION OF WHITE SPOT SYNDROME VIRUS AND TAURA SYNDROME VIRUS BY MULTIPLEX PCR

摘要: 正对虾白斑综合征(WSS)和桃拉病(TS)是两种严重危害当前对虾养殖业的对虾病毒性疾病。目前对虾病毒病的诊断手段主要包括病理学和生物学方法、免疫学方法、分子杂交方法及PCR方法[1-4]等。其中PCR方法是这些方法中特异性最强、敏感最高的病原检测手段,在国外已被广泛应用于对虾病毒病的检测和诊断。多重PCR是一种特殊PCR形式,其最突出特点,即一次PCR反应,就可同时检测、鉴别出多种病原体,在临床混合感染的鉴别诊断上具有其独特优势和很高的实用价值[5,6]。本试验建立了二温式多重PCR同时检测鉴别WSSV和TSV的方法,并用该多重PCR方法对广西沿海地区对虾养殖业WSSV和TSV感染状况进行了初步调查。现将结果报告如下。
- 多重聚合酶链式反应 /
- 检测 /
- 对虾 /
- 白斑综合征病毒(WSSV) /
- 桃拉病毒(TSV)
- Multoplex Polymerase Chain Reaction /
- Detection /
- White Spot Syndrome Virus /
- Taura Syndrome Virus

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