铁-锰氧化鞘细菌的生境条件及分离鉴定

ISOLATION IDENTIFICATION AND HABITAT CONDITIONS OF Fe-Mn OXIDIZED SHEATHED BACTERIA

  • 摘要: 从水库采集不同季节和不同水层的水样及水下输水管道堵塞物进行分析和分离,结果表明,铁-锰氧化鞘细菌分布在水库不同水层的低浓度有机营养的水流和水下输水管道的堵塞物中。该菌在水库中较适的生境条件在夏秋两季,温度为21-25℃、pH6.16-6.87,总铁含量>0.43mg/L和微氧环境的中下层及底层。该菌的分离采用富集培养和限制营养浓度的选择培养基。富集鞘细菌以尿素-葡萄糖-无机盐培养基和Stokes培养基为较有效培养基,富集和分离铁-锰氧化鞘细菌以Winogradsky铁细菌培养基和Muder锰培养基为有效培养基,添加0.5u/mL青霉素钠可提高铁-锰氧化鞘细菌的出菌率,可选择CGY培养基进一步纯化。分离到2株:FC771-1和FC9771-2,经形态和培养特征的初步鉴定,FC9771-1属球衣菌属(Sphaerotilus),FCP771-2属于纤发菌属(Leptothrix).

     

    Abstract: The water samples obtained in different water layers of a reservoir and inplugs of water pipes were collected for the investigation of Fe-Mn oxidized sheathedbacteria The bacteria were found in plugs and the layers where the organic nutritionwas considered low. It is indicated that the best seasons for the growth of bacteriaare summer and autumn, and the suitable growing place is in the lower layer andbottom of the reservoir where the temperature is 21-25℃, pH 6.16-6.87 Fe 0.43mg / L, and oxygenic concentration is low. The selective media with limitednutrient concentrations and enriched culture were used for isolation of the bacteria.The results showed that urea-glucose-inorganic salt medium and Stokes medium aresuitable for the enrichment of the sheathed bacteria and Winogradsky iron bacteriamedium and Muder managanese medium are more effective for the enrichment andisolation of Fe-Mn oxidized sheathed bacteria. The addition of 0.5u/ml of sodiumpenicilline in the medium can increase the growth rate of Fe-Mn oxidized sheathedbacteria CGY medium can be used to purify the bacteria; Two strains of Fe-Mnoxidized sheathed bacteria,FC9771-1 and FC9771-2, were finally purified. Accordingto their morphological and culture features, FC9771-1 was preliminarily identified asgenus in the Sphaerotilus, FC9771-2 as Leptothrix genus.

     

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