Abstract: Plants have many endogenous genetic, biochemical and physiological properties that make the mideal tools for soil and water remediation. The genetic engineering plants open up the new possibilities for the phytorem ediation. Pistia stratiotesm ay be a useful plant in remediation of water pollutants by transgenic engineering. Pistia stratiotes is a widely distributed free-floating perennial hydrophyte. Because a large number of organism s are associated with non-axenicPistia stratiotes, it is miportant to obtain the axenic culturematerials of Pistia stratiotes. So we have developed axenic Pistia cultural system and studied the effects of phytohormone, sucrose and pH on its growth. The results showed that both phytohorm one and sucrose have significant effects on the grow th of cultured Pistia stratiotes, while pH has little effect. The optmi ized culture system was determined as follow ing: 1/2MS+ 6-BA 0125mg /L + NAA 013mg /L + sugar20g/L and pH6. 0. The axenic Pistia stratiotes was comparable to the greenhouse-grown plants except for the smaller size.These results will provide a usefulbasis for genetic transformation ofPistia stratiotes for the remediation ofwater pollutants