中华鳖4个Sox基因保守区的序列分析
THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS
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摘要: 采用PCR技术,扩增和克隆了中华鳖Sox基因(TSSox).经DNA序列分析显示,Sox基因在系统进化上十分保守,其中TSSox4与鸟类LF4基因编码的氨基酸序列完全相同、与人类SOX4和小鼠Sox4编码的序列仅一个氨基酸的差异;TSSox5与鸟类的LF5基因的编码也仅一个氨基酸发生了改变;TSSox2与海龟的TSox2相似性最高.4条TSSox序列中,TSSox与人SRY基因序列相似性最高,达75%;序列上的相似性可能暗示了它们在功能上的保守性Abstract: The Sox genes of Trionyx sinensis were amplified and cloned using PCR. The four different fragments of Sox gene HMG-box DNA were selected and sequenced. The results indicated that the Sox gene is conservative in phylogeny, in which the amino acid sequence encode by TSSox4(The Sox of T. Sinensis)is identical to those encode by LF4(bird)or differ from SOX4(human)and Sox4(mouse)only on one amino acid; The amino acid sequences encode by TSSox5 have an amino acid difference from those encode by LF5(bird); TSSox1 has about 75% identical to SRY HMG-box at the nucleotide level, and is most resemblance among four TSSox. Phylogenetic analysis of available 32 Sox sequences including SRY/Sry sequence suggests that there wolud be a high degree of divergence between any possible immediate common ancestor of the T. Sinensis Sox sequence and the SRY(Sry)sequences.
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Keywords:
- Trionyx sinensis /
- Sox gene /
- Sequence analysis /
- PCR
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[1] Janzen J F.Environmental sex determination in reptiles: Ecology,Evolution,and Experimental design[J].The Quarterly Review of Biology,1991,66(2):149—179[2] Spotile L D,Kaufer N F,Theriot E.Sequence analysis of the ZFY and sox genes in the turtle,chelydra serpentina[J].Molecular Phylogenetic and Evolution,1994,3(1):1—9[3] Western P S,Harry J L,Graves J.a,et al.Temperature-dependent sex determination: upregulation of SOX9 expression after commitment to male development[J].Development-Dynamics,1999,214(3):171—177[4] 聂刘旺,郭超文,汪鸣.中华鳖性别决定机制的研究[C],安徽省第三届“兴皖之光”青年学术年会论文集合肥;安徽科技出版社,1998,59—64[5] 聂刘旺,单祥年,郭超文.两种龟类Sox基因的PCR扩增及SSCP分析的研究[J].应用与环境生物学报 1999,5(4);378—381[6] Sambrook J,Frisch E F,Maniatis T.Molecular cloning—a laboratory manual(2 nd ed)[M] Cold Spring Harbor Laboratory Press.1989,[7] Capel B.Sex in the 90s: SRY and the switch to male pathway[J].Annu.Rev.Physial.1998,60:497—521[8] Elreavey K.Vilain E.Kaufer NF.et al: A regular cascade hypothesis for mammalian sex determination: SRY represses a negative regulator of male development[J].PNAS of USA,1993,90:3368[9] Vilain E,McCobe E.R Mammalian sex determination: From gonads to brain[J].Mol.Genet.Metab.1998,65(2):74—84[10] Schilham MW,Moerer P,Cumano A.HC Clevers.,Sox4 facilitates thymocyte differentiation[J].European Journal of Immunology,1997,27(5):1292—1295[11] Ahn S G.,Cho G H.,Jeong S Y.et al.Identification of cDNAs for Sox4,an HMG-Box＼protein,and a novel human homology of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/deltal2-PGJ2 in Hep3B cells[J] Biochemical And Biophysical Research Communications,1999,260(1):216—221 Janzen J F.Environmental sex determination in reptiles: Ecology,Evolution,and Experimental design[J].The Quarterly Review of Biology,1991,66(2):149—179[2] Spotile L D,Kaufer N F,Theriot E.Sequence analysis of the ZFY and sox genes in the turtle,chelydra serpentina[J].Molecular Phylogenetic and Evolution,1994,3(1):1—9[3] Western P S,Harry J L,Graves J.a,et al.Temperature-dependent sex determination: upregulation of SOX9 expression after commitment to male development[J].Development-Dynamics,1999,214(3):171—177[4] 聂刘旺,郭超文,汪鸣.中华鳖性别决定机制的研究[C],安徽省第三届“兴皖之光”青年学术年会论文集合肥;安徽科技出版社,1998,59—64[5] 聂刘旺,单祥年,郭超文.两种龟类Sox基因的PCR扩增及SSCP分析的研究[J].应用与环境生物学报 1999,5(4);378—381[6] Sambrook J,Frisch E F,Maniatis T.Molecular cloning—a laboratory manual(2 nd ed)[M] Cold Spring Harbor Laboratory Press.1989,[7] Capel B.Sex in the 90s: SRY and the switch to male pathway[J].Annu.Rev.Physial.1998,60:497—521[8] Elreavey K.Vilain E.Kaufer NF.et al: A regular cascade hypothesis for mammalian sex determination: SRY represses a negative regulator of male development[J].PNAS of USA,1993,90:3368[9] Vilain E,McCobe E.R Mammalian sex determination: From gonads to brain[J].Mol.Genet.Metab.1998,65(2):74—84[10] Schilham MW,Moerer P,Cumano A.HC Clevers.,Sox4 facilitates thymocyte differentiation[J].European Journal of Immunology,1997,27(5):1292—1295[11] Ahn S G.,Cho G H.,Jeong S Y.et al.Identification of cDNAs for Sox4,an HMG-Box＼protein,and a novel human homology of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/deltal2-PGJ2 in Hep3B cells[J] Biochemical And Biophysical Research Communications,1999,260(1):216—221
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