日本产和中国产中华细鲫的RAPD分析

基金项目:

国家自然科学基金课题(批准号:39500016)

THE RAPD ANALYSIS FOR CYPRINID FISH(APHYOCYPRIS AND HEMIGRAMMOCYPRIS)

Institute of hydrobiology, The Chinese Academy of Sciences, Wuhan 430072;
2. Lake Biwa Museum, Shiga, 525-001, Japan

摘要: 使用20个随机引物对日本和中国辽宁产中华细鲫、日本产Hemigrammocypris sp.和中国产稀有鲫各4个个体的随机扩增,得到了大量DAN多态片段.通过Rapdplot程序将DAN的多态片段转换成遗传距离(d=1-S,S=2NxNy/Nx+Ny).该遗传距离的矩阵经Phylip软件包中Neighbor程序计算,生成了四个物种的相互关系分支图.从图中可以看出,分布于日本和中华细鲫有较近的遗传距离,然后是Hemigrammocypris,最后是稀有鲫.该结果说明分布于日本和中国的中华细鲫是同一个物种.日本的Hemigrammocypris是一个独立的物种.
- 鲤科鱼类 /
- 亲缘关系 /
- RAPD
Abstract: Many informative fragment of RAPD were obtained from Aphyocypris chinensis(Japan and China), Hemigrammocypris sp.(Japan), Gobiocypris rarus(China)by the method of random amplifing with 20 primers. We convert the DNA polymorphis fragment into genetic distance by the program Rapdplot(d=1-S, S=2NxNy/Nx+Ny). The matrix of genetic distance was caculated by the program Neighbor in software package Phylip, resulting in one cladogram of four species. This result indicated that the species Hemigrammocypris sp. is an independent one and the species Aphyocypris in China and Japan is the same species.
- Cyprinidae /
- Phylogeny /
- RAPD

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