中华鳖病毒的血清学检测
SEROLOGICAL DETECTION FOR THE TRIONYX SINENSIS VIRUS
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摘要: 中华鳖病毒(TSV)是从病鳖中分离到的一种病毒病原.经细胞培养和差异离心制备TSV抗原,肌注家兔获TSV抗体(TSV-Ab),中和效价为1:20.用TSV-Ab进行双向免疫扩散和间接ELISA检测,被检样品有健康和病鳖组织匀浆液、TSV细胞培养液,提纯的TSV,以及鱼传染性胰脏坏死病毒(IPNV)、草鱼呼肠孤病毒(GCV)、鱼病毒性出血败血症病毒(VHSV)、鲤痘疮病毒(Carp pox virus)和斑节对虾杆状病毒(MBV).检测结果显示:IPNV、GCV、VHSV、Carp pox virus、MBV和健康中华鳖的组织匀浆液与TSV-Ab呈阴性反应;而感病中华鳖组织匀浆液、TSV感染的细胞提取液与TSV-Ab呈阳性反应.Abstract: Trionyx sinensis virus (TSV) was isolated from the lethal disease Chinese soft-shelled turtle.Antibody to the virus (TSV-Ab) has been produced from rabbits raised to TSY that was purified from infected EPC cells.The neutralization bile of TSV-Ab was l;20.The TSV-Ab was applied to immunodiffusion and indirect ELISA for the virus pathogen detection.The detected samples included the homogenate of the health and diseased turtle tissues, TSV infected cell extracts, purified TSV, and other viruses from aquatic animals.Immunodiffusion and ELISA results by the TSV-Ab indicated that infectious pancreatic necrosis virus (IPNV), grass carp reovirus (GCV), viral haemorrhagic septiaemia virus (VHSV), carp pox virus, monodon baculovirus (MBV) and the homogenate of health turtle tissues were seronegative, while the infected cell extracts and the homogenate of diseased turtle tissues were seropositive.Thus, the immunodiffusion and the ELISA test were easy and sensitive methods for detecting TSV pathogen in the turtle aquaculture.
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