圆形碘泡虫和关桥碘泡虫孢子的光镜及扫描电镜观察
STRUCTURE OF THE SPORES OF MYXOBOLUS ROTUNDUSAND AND MYXOBOLUS GUANQIAOENSIS UNDER OPTICAL AND SCANNING ELECTRON MICROSCOPY
-
摘要: 对寄生于鲫的圆形碘泡虫(Myxobolus rotundus Nemeczek, 1911)及异育银鲫的关桥碘泡虫(M. guanqiaoensis Wu & Wang, 1997)的成熟孢子进行光镜及扫描电镜观察.圆形碘泡虫主要寄生于鲫体表、头部、鳃、吻部及鳍条,形成许多大小不一的乳白色胞囊.关桥碘泡虫主要寄生于异育银鲫的肝脏,肝脏基本上被虫体所充满,肝脏组织被破坏.扫描电镜观察表明圆形碘泡虫成熟孢子表面光滑,缝脊直而明显,宽0.2μm,缝脊上下两侧对称.关桥碘泡虫成熟孢子表面皱褶,其程度不等,有的虫体一侧向内凹陷形成盆状,缝脊呈"S"形,宽0.μm,缝脊上下两侧不对称,其中间有一不很明显凹陷的沟.Abstract: This paper dealt with the structure of the mature spore of Myxobolus rotundus Nemeczek, 1911 and Myxobolus guanqiaoensis Wu Wang, 1997 under the optical and scanning electron microscopy. M. Rotundus was found in the body surface, head, gill, lip, and fin of Carassius auratus . It formed various inch cysts. M. guanqiaoensis was found in the liver of allogynogenetic silver crucian carp. It was found by histopathological observations that heavy infection of the spores could destroy the hepatic tissue.Under scanning electron microscopy, surface of the spore of M. rotundus was generally smooth. Its sutural line was straight and distinctive, running near the middle of the valves. Width of the sutural ridge was about 0.2 μm. Surface of the spore of M. guanqiaoensis was lightly shrunk. Its sutural line was S shaped and about 0.5 μm in width. The both valves of the spore were not bilateral symmetry. A furrow was observed in middle of sutural ridge. No striation pattern was found on surface of the spore, and no mucous envelope was around the spore in the two species.
-
-
[1] Lom J, Myxosporea: a new look at long-known parasites of fish[J]. Parasitol. Today, 1987,3(11) :327-332[2] Chen Q L, Ma C L. Fauna sinica Myxozoa: Myxosporea[M]. Beijing: Science Press. 1998.[陈启鎏,马成伦.粘孢子虫志.北京:科学出版社, 1998][3] Pan J P. Handbook of the diagnoses and the prophylaetics and therapeutics of fish diseases[M]. Shanghai scientific and technical publishers [潘金培. 鱼病诊断与防治手册.上海:上海科学技术出版社,1988,5-8][4] Wu Y S and Wang J G. A new species of myxosporidian from Allogynogenetic silver crucian carp (Myxosporea: Bivalvulida, Myxobolidae)[J]. Acta Hydrobiologica Sinica, 1997, 21(3):268-270.[吴英松,汪建国.寄生异育银鲫的碘泡虫一新种(粘孢子纲:双壳目,碘泡科).水生生物学报, 1997,21(3):268-270][5] Nemecaek A. Beitrage zur Kenntnis der Myxo-und Microsporidien der Fish [J]. Archivfur Protistenkunde, 1911, 22: 143-169[6] Liu L Q, Wang B D, Yang T. Studies on myxosporidia from Gymnocypris przewalskii przewlaskii (Kessler) in Qinghai Lake[J]. Acta Biologica Plateau Sinica, 1982, 61-68.[刘立庆,王宝铎,杨涛.青海湖裸鲤寄生粘孢子虫的研究.高原生物学集刊, 1982,(1):61-68][7] Lom J, Hoffman G L, Morphology of the spores of Myxosoma cerebralis (Hofer,1903) and M.cartilaginis (Hoffman, Putz, and Dunbar, 1965) [J]. J. Parasitology, 1971, 57:1302-1308[8] Desser S S, Paterson W B, Ultrastructural and cytochemical observations on sporogenesis of Myxobolus sp. (Myxosporida:myxobolidae) from the common shiner Notropis cornutus [J]. J. Protozool, 1978, 25:314-326[9] Mitchell L G, et al. Light and electron microscopy of Myxobolus hendricksoni sp. nov. (Myxozoa: Myxobolidae) infecting the brain of the fathed minnow, Pimephales promelas Rafinesque[J]. J. Fish. Dis, 1985, 8:75-89[10] Hine P M, A review of some species of Myxidium Butschli, 1882 (Myxozossporea) from eels (Anguilla spp.)[J]. J. Protozool, 1980, 27(3):260-267 Lom J, Myxosporea: a new look at long-known parasites of fish[J]. Parasitol. Today, 1987,3(11) :327-332[2] Chen Q L, Ma C L. Fauna sinica Myxozoa: Myxosporea[M]. Beijing: Science Press. 1998.[陈启鎏,马成伦.粘孢子虫志.北京:科学出版社, 1998][3] Pan J P. Handbook of the diagnoses and the prophylaetics and therapeutics of fish diseases[M]. Shanghai scientific and technical publishers [潘金培. 鱼病诊断与防治手册.上海:上海科学技术出版社,1988,5-8][4] Wu Y S and Wang J G. A new species of myxosporidian from Allogynogenetic silver crucian carp (Myxosporea: Bivalvulida, Myxobolidae)[J]. Acta Hydrobiologica Sinica, 1997, 21(3):268-270.[吴英松,汪建国.寄生异育银鲫的碘泡虫一新种(粘孢子纲:双壳目,碘泡科).水生生物学报, 1997,21(3):268-270][5] Nemecaek A. Beitrage zur Kenntnis der Myxo-und Microsporidien der Fish [J]. Archivfur Protistenkunde, 1911, 22: 143-169[6] Liu L Q, Wang B D, Yang T. Studies on myxosporidia from Gymnocypris przewalskii przewlaskii (Kessler) in Qinghai Lake[J]. Acta Biologica Plateau Sinica, 1982, 61-68.[刘立庆,王宝铎,杨涛.青海湖裸鲤寄生粘孢子虫的研究.高原生物学集刊, 1982,(1):61-68][7] Lom J, Hoffman G L, Morphology of the spores of Myxosoma cerebralis (Hofer,1903) and M.cartilaginis (Hoffman, Putz, and Dunbar, 1965) [J]. J. Parasitology, 1971, 57:1302-1308[8] Desser S S, Paterson W B, Ultrastructural and cytochemical observations on sporogenesis of Myxobolus sp. (Myxosporida:myxobolidae) from the common shiner Notropis cornutus [J]. J. Protozool, 1978, 25:314-326[9] Mitchell L G, et al. Light and electron microscopy of Myxobolus hendricksoni sp. nov. (Myxozoa: Myxobolidae) infecting the brain of the fathed minnow, Pimephales promelas Rafinesque[J]. J. Fish. Dis, 1985, 8:75-89[10] Hine P M, A review of some species of Myxidium Butschli, 1882 (Myxozossporea) from eels (Anguilla spp.)[J]. J. Protozool, 1980, 27(3):260-267
计量
- 文章访问数: 1148
- HTML全文浏览量: 1
- PDF下载量: 453
下载: