重口裂腹鱼消化道内分泌细胞的免疫组织化学鉴别和定位
LOCALIZATION AND IDENTIFICATION OF ENDOCRIN CELLS IN THE DIGESTIVE TRACT OF SCHIZOTHORAX DAVIDI
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摘要: 重口裂腹鱼(Schizothoraxdavidi)俗称“雅鱼”,属鲤科,裂腹鱼亚科,主要分布于大渡河、青衣江水系上游,以动物性食性为主。因其生长缓慢,肉质鲜嫩,深受人们喜爱。随着捕捞强度的增大和自然生态环境的破坏,其资源量急剧下降。目前大多数学者使用哺乳动物抗血清对鱼类消化道内分泌细胞进行了鉴别和定位,在硬骨鱼和软骨鱼的胃肠胰中分别发现了17种和20种内分泌细胞[1]。本研究采用链霉亲和素-生物素过氧化物酶(Strept Avidin-Biotin-Complex,SABC)免疫组织化学技术对重口裂腹鱼消化道胃泌素(Gastrin,Gas)、5-羟色胺(5-hydroxytryptamine,5-HT)、生长抑素(Somatostatin,Som)、胰高血糖素(Glucagon,Glu)内分泌细胞进行了鉴别和定位。因此,这项研究可为鱼类组织学、消化生理及内分泌学研究领域提供新资料,同时也为开发研究重口裂腹鱼的人工配合饲料,进行规模化养殖等提供参考。
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Keywords:
- Schizothorax davidi /
- Digestive tract /
- Endocrine cell /
- Immunocytochemistry
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[1] He M, Fang J.Perspective of secretory cells of digestive tracts in fishs [J].Fisheries Science, 2005, 24(6):46-49[何敏, 方静.鱼 类消化道内分泌细胞的研究进展.水产科学, 2005, 24(6): 46-49]
[2] Xi a L Q, Wang Q T.Immunoctyochemical study of the endocrine cells in the gastrointerstinal tract of Ophiocephalus argus [J].Zoological Research, 1994, 15(4):34-35[夏立群, 王庆堂.乌鳢胃 肠内分泌细胞的免疫组织化学研究.动物学研究, 1994, 15 (4):34-35]
[3] Lin S G.Huang X H, Wu D F.Localization and identification of endocrine cells in the alimentary canal of Lateolabrax japonicus [J]. Acta Hydrobiologica S inica, 2005, 29(1):7-9[林树根, 黄小华, 吴德峰.花鲈消化道内分泌细胞的鉴别和定位.水生生物学 报, 2005, 29(1):7-9]
[4] Lin S G, Wu D F, Wang S K, et al.Locali zation and identifi cation of endocrine cel ls in the gastro-entero pancreas of Angui lla japonicus[J].Journal of Fisheries of China, 2003, 27(6):513-518[林树 根, 吴德峰, 王寿昆, 等.日本鳗丽胃肠胰内分泌细胞的鉴别与 定位.水产学报, 2003, 27(6):513-518]
[5] Fang Y Q, Weng Y Z, Hu X X, et al.Immunohistochemical localization of endocrine cells in gast rointestinal tract of Mugi l cephalus[J].Journal of Fisheries of China, 2002, 26(6):481-485[方永 强, 翁幼竹, 胡晓霞, 等.鲻鱼胃肠道内分泌细胞免疫组织化学 的定位.水产学报, 2002, 26(6):481-485]
[6] Fang Z P, Pan Q S, Nie X Y, et al.Locali zation and identifi cation of endocrine cells in the gastro-entero-pancreatic system of two species of stomachcontaning teleosts [J].Journal of Huazhong Agricultural University, 1997, 16(6):594-598[方之平, 潘黔生, 聂秀云, 等.两种有胃真骨鱼胃肠胰系统中内分泌细胞的鉴别与定位. 华中农业大学学报, 1997,16(6):594-598]
[7] Pan Q S, Fang Z P, Fan Q X, et al.Immunohi stochemical localization and morphological study on G cells in the intestion of Hypophthalmichthys Molitrix, Aristichthys Nobilis, Carassius Auratus Gibelio and Megalobrama Amblyocephala [J].Acta Zoologica Sinica, 1995, 41(2):167-171[潘黔生, 方之平, 樊启学, 等, 鲢、鳙、银鲫、团 头鲂肠道G 细胞定位与免疫组化研究.动物学报, 1995, 41(2):167-171]
[8] Pan Q S, Fang Z P.The immunohi stochemical location and comparison of gastrinimmunoreative cells in the intestinal tract of four carps species [J].Journal of Huazhong Agricultural University, 1988, 7(3):238-242[潘黔生, 方之平.四种鲤科鱼肠道中胃泌素免 疫活性细胞的免疫组织化学定位及比较.华中农业大学学报, 1988, 7(3):238-242]
[9] Pan Q S, Fang Z P, Zhao Y X.Immunocytochemical identification and locali zation of APUD cells in the gut of seven stomachless teleost f ishs [J].World Journal of Gastroenterology, 2000, 6(1):96-101
[10] Noailac-Depeyre J, Hollande E.Evidence for somatostation, gastrion and pancreatic polypeptide-like substances in the mucosa cel ls of the gut in fishes with and without stomach [J].Cell Tiss.Res., 1981, 216:193-203
[11] Rombout J H W M, Taverne-Thiele J J.An immunocytochemi caland electron microscopi cal study of endocrine cells in the gut and pancreas of a stomachless teleost fish, Barbus conchonius (Cyprinidae)[J]. Cell Tiss Res., 1982, 227:577-593
[12] Pan Q S, Fang Z P.The immunocytochemical evidence for four hormones in the gastrointest inal endocrine cells of mandarin f ish [J]. Acta Hydrobiologica S inica, 2001, 25(6):565-570[潘黔生, 方 之平.几种激素在鳜鱼胃肠道内分泌中存在的免疫细胞化学 证据.水生生物学报, 2001, 25(6):565-570]
[13] Wang Z J.Gut hormone[M].Beijing:Beijing Science Press.1985, 1-6[王志均.胃肠激素.北京:北京科学出版社.1985, 1-6]
[14] Solcia E, Capella.Endocrine cells of the gastric mucosa [J].Int Rev Cytol, 1975, 42:223-286
[15] Shu M A, Xu H S, Zhu Y F, et al.Somatostatin secreting cells and ultrastructure of stomach and intestine of Monopterus albus [J]. Journal of Fisheries of China, 2002, 26(3):206-212[舒妙安, 徐 海圣, 朱有法, 等.黄鳝胃肠的生长抑素分泌细胞及超微结构. 水产学报,2002, 26(3):206-212]
[16] Fang Z P, Junzo Yamada, Pan Q S.Localization and identifi cation of endocrine cells in the intestinal mucosa of common carp and black carp [J].Acta Hydrobiologica Sinica, 1991, 15(3):212-217 [方 之平, 山田纯三, 潘黔生, 鲤、青鱼肠黏膜内分泌细胞的免疫 组织化学鉴别和定位.水生生物学报, 1991, 15(3):212-217]
[17] Pan Q S, Fang Z P.Present progress in the study of the APUD cells in gastro-entero-pancreati c endocrine system of the fishes [J].Acta Hydrobiologica Sinica, 1995, 19(3):275-282[潘黔生, 方之平. 鱼类胃肠胰内分泌系统APUD 细胞研究的现状.水生生物学 报,1995, 19(3):275-282]
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