Abstract: The properties of proteinases in the tissues of goldfish and the kinds of proteinases in the embryogenesis of goldfish were investigated with G-PAGE. The kinds of proteinases were similar, and most tissues of goldfish had four kinds of proteinases with molecular weights being 113 kD, 69 kD, 20 kD and 16 kD respectively.Many kinds of proteinases with strongest activities were observed in the gut. The activities of proteinases were affected by pH, and the optimum pH was 8.5. In the embryogenesis of goldfish, the proteinases began to express at hatching stage, but not in the early embryogenetic period. The proteinases started to express at hatching stage,but most of them expressed at the late developmental stage.