Abstract: The movement of Chinese sturgeon spermatozoon in various concentration of Na+,K+,Ca2+ ,Mg2+ ,Cu2+ and glucose , fructose solution was studied in this paper.The results showed that the longest lifetime was 347s when the concentration of Na+ was 2 ‰,while the longest acute movement time was 98.67s when the concentration of Na+ was 1 ‰.The activation rate of sperm was 100 % when the concentration was no more than 2 ‰1Compared with Na+ solution ,when the concentration of K+ was 01005 ‰,the motility of spermwas the highest ,while the acute movement time and lifetime were the longest ,which were 80s and 174s1The activation rate increased with the increase of the concentration of K+ When the concentration was 01005 ‰,the motility was the highest ,which was 60 %.And then it decreased sharply.In the three solution of Ca2+ ,Mg2+ and Cu2+ ,Along with the increase of the concentration of Ca2+ ,Mg2+ and Cu2+ ,both the acute movement time and lifetime decreased ,and the activation rate of sperm also decreased gradually.The situation in the solution of glucose and fructose was absolutely different fromthe above 3 solution1Along with the increase of the concentration of glucose and fructose ,the acute movement time and lifetime increased gradually.The results indicated that the appropriate concentration of Na+ and K+ could increase the acute movement time and lifetime ,and activated the sperm,while Ca2+ ,Mg2+ and Cu2+ under low concentration had obvious inhibiting effect on the acute movement time,lifetime and activation rate; while glucose and fructose can effectively increased the acute movement time and lifetime of sperm1.