两种裸藻的无叶绿体突变株
CHLOROPLAST-LESS MUTANTS OF TWO SPECIES OF EUGLENA
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摘要: 利用Ofloxacin处理获得了纤细裸藻和中型裸藻无叶绿体突变株.吸收光谱测定和自荧光显微观察证明突变株无叶绿素合成,DAPI染色方法显示突变株细胞不存在质体DNA,而无色的长变胞藻细胞中仍有可检测质体DNA,说明二突变株质体已消失.SDS-PAGE显示野生种和突变株各有特异蛋白表达.Abstract: Chloroplast less mutants of E. gracilis and E. intermedia were generated by treatment with Ofloxacin Absorption spectra and autofluorescence microscopic observations showed that chlorophyll was not synthesized in the two mutants Using DAPI fluorescence photomicrograph, plastid DNA was found in Astasia longa, a colorless species of Euglenoid, but not in the ofloxacin induced mutants of Euglena, which suggest that plastids have been completely lost in the two mutants SDS-PAGE indicated that specific proteins were synthesized in the wild type and mutant strains respectively
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Keywords:
- Euglena /
- Chloroplast /
- Mutants
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[1] John L Mego. The Biology of Euglena[M].New York:Academic Press,1968[2] Osafune T,Jerome A.Schiff. W10BSmL, a mutant of Euglena gracilis var. bacillaris lacking plastids[J].Exp Cell Res, 1983,148:530-535[3] Krajcovic L,Chreno O,Ebringer L. Different effect of hyperthermia and heat shock on the action of quinolone drugs versus some mutagents against chloroplasts of Euglena gracilis[J].Mutation Res,1990,244:21-25[4] Krizkova L,Nagy M,Polonyi et al. The effect of flavonoids on ofloxacininduced mutagentcity in Euglena graiclis [J].Mutation Res,1998,416:85-92[5] Krizkova L,Nagy M,Polonyi J,et al Phenolic acids inhibit chloroplast mutagenesis in Euglena gracilis[J].Mutation Res,2000,469:107-114[6] 潘瑞炽,董愚得.植物生理学,第3版[M].北京:高等教育出版社,1995[7] Rowan K S. Photosynthetic pigments of algae[M].Cambridge:Cambridge University Press,1989[8] Siemeister G,Hachtel W.Organization and nucleotide sequence of ribosomal RNA genes on a circular 73kbp DNA from the colourless flagellate Astasia longa[J].Current genet,1990,17:433-438 John L Mego. The Biology of Euglena[M].New York:Academic Press,1968[2] Osafune T,Jerome A.Schiff. W10BSmL, a mutant of Euglena gracilis var. bacillaris lacking plastids[J].Exp Cell Res, 1983,148:530-535[3] Krajcovic L,Chreno O,Ebringer L. Different effect of hyperthermia and heat shock on the action of quinolone drugs versus some mutagents against chloroplasts of Euglena gracilis[J].Mutation Res,1990,244:21-25[4] Krizkova L,Nagy M,Polonyi et al. The effect of flavonoids on ofloxacininduced mutagentcity in Euglena graiclis [J].Mutation Res,1998,416:85-92[5] Krizkova L,Nagy M,Polonyi J,et al Phenolic acids inhibit chloroplast mutagenesis in Euglena gracilis[J].Mutation Res,2000,469:107-114[6] 潘瑞炽,董愚得.植物生理学,第3版[M].北京:高等教育出版社,1995[7] Rowan K S. Photosynthetic pigments of algae[M].Cambridge:Cambridge University Press,1989[8] Siemeister G,Hachtel W.Organization and nucleotide sequence of ribosomal RNA genes on a circular 73kbp DNA from the colourless flagellate Astasia longa[J].Current genet,1990,17:433-438
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