集胞藻PCC6803中ORF sll0260对于维持基本生命活动的必要作用

罗泉; 徐旭东

集胞藻PCC6803中ORF sll0260对于维持基本生命活动的必要作用

罗泉^1,2,
徐旭东^1, ,

中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉 430072
2. 中国科学院研究生院, 北京 100049

基金项目:

国家自然科学基金重点项目(30330030)

中国科学院知识创新工程方向性项目(KSCX2-SW-332)资助

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出版历程
- 收稿日期: 2008-05-12
- 修回日期: 2009-01-12
- 发布日期: 2009-09-24

THE ESSENTIAL ROLE OF ORF SLL0260 INMA INTENANCE OF BASIC L IFE ACTIVITIES OF SYN ECHOCYSTIS SP1 PCC6803

LUO Quan^1,2 and
XU Xu-Dong^1, ,

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072;
2. The Graduate School of Chinese Academy of Sciences, Beijing 100049

摘要

摘要: 集胞藻(Synechocystis sp.)6803的未知功能基因中有很多是细胞的基本生命活动所需要的,这些基因插入失活往往会导致细胞死亡,因而得不到分离完全的突变株,难以进行遗传学研究。构建突变株以铜离子调控的启动子PpetE来控制此类未知功能基因的表达则可能获得完全分离。构建PpetE-sll0260突变株并对sll0260必要作用进行研究。在完全分离的突变株中,去除铜离子可关闭sll0260的表达。此时,突变株生长受到严重抑制,色素含量大为降低,类囊体膜结构破坏,光合作用消失,呼吸能力下降。这些结果表明该基因对于维持集胞藻6803的基本生命活动来说是必需的。亚细胞定位研究显示sll0260编码一个膜蛋白,位于质膜和外膜混合物中。Sll0260可能作为某些离子的转运蛋白起作用,或者直接与类囊体膜的发生过程相关。
- 集胞藻680 /
- sll0260 /
- PpetE /
- 膜蛋白
- Synechocystis sp. PCC6803 /
- sll0260 /
- PpetE /
- Membrane protein

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参考文献(19)

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集胞藻PCC6803中ORF sll0260对于维持基本生命活动的必要作用

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出版历程

THE ESSENTIAL ROLE OF ORF SLL0260 INMA INTENANCE OF BASIC L IFE ACTIVITIES OF SYN ECHOCYSTIS SP1 PCC6803

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出版历程

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