嗜热四膜虫接合生殖周期皮层骨架蛋白组分的比较
STUDIES ON CORTICAL CYTOSKELETAL PROTEINS IN IMPORTANT PHASES DURING CONJUGATION OF TETRAHYMENA THERMOPHILA
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摘要: 嗜热四膜虫(Tetrahymena thermophila)BF株BF1、BF5系细胞为材料,根据显微观察将其接合生殖周期分为四个特定的阶段,采用生化抽提和SDSPAGE及扫描、数据统计,分析了营养期与接合生殖前期、中期、后期、末期同类蛋白质组成.发现80KD、87KD和88KD仅在营养期和接合前期;而905KD、855KD和66KD则存在于接合生殖的各时期.这些蛋白的缺失与出现,可能与小核的减数分裂、合子的形成及分裂、接合区膜的形成有着某种联系.
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关键词:
- 嗜热四膜虫 /
- 接合生殖 /
- 皮层细胞骨架蛋白 /
- SDS-聚丙烯酰胺凝胶电泳
Abstract: To study the changes of cortical cytoskeletal proteins of Tetrahymena thermophila, we used the cells of mating type-Tetrahymena thermophila BF1 and BF5. When mixed, the cells underwent a sexual phase involing conjugation. The conjugation course was divided into four important phases in accordance with the changes of cellular nucleuses and their form with microscopic examination. Cortical cytoskeleton proteins had been prepared by biochemical extraction with Tritonx-100, SDS-PAGE and scan. The data statistics had been analyzed by a computer. The results showed that 55KD, 52 5KD, 49KD and 225KD, 150KD, 130KD decreased during conjugation. The 80KD, 87KD, 88KD only appeared in vegetation and early of conjugation, but 90 5KD, 85 5KD and 66KD appeared in the whole conjugation course. These proteins were involved in cortical reorganization during conjugation. They also had some connections with the meisosis of small nucleus and the formation of conjugation fileds.-
Keywords:
- Tetrahymena thermophila /
- Conjugation /
- Cortical cytoskeletal proteins /
- SDS-PAGE
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