南方鲇生长激素完整cDNA的克隆及其DNA序列分析

宋平, 向筑, 潘云峰, 胡隐昌, 胡珈瑞

宋平, 向筑, 潘云峰, 胡隐昌, 胡珈瑞. 南方鲇生长激素完整cDNA的克隆及其DNA序列分析[J]. 水生生物学报, 2002, 26(3): 272-280.
引用本文: 宋平, 向筑, 潘云峰, 胡隐昌, 胡珈瑞. 南方鲇生长激素完整cDNA的克隆及其DNA序列分析[J]. 水生生物学报, 2002, 26(3): 272-280.
SONG Ping, HU Yin-chang, XIANG Zhu, HU Jia-rui, PAN Yun-feng. CLONING AND SEQUENCING OF FULL LENGTH cDNA IN SILURUS MERIDIONALIS GROWTH HORMONE[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(3): 272-280.
Citation: SONG Ping, HU Yin-chang, XIANG Zhu, HU Jia-rui, PAN Yun-feng. CLONING AND SEQUENCING OF FULL LENGTH cDNA IN SILURUS MERIDIONALIS GROWTH HORMONE[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(3): 272-280.

南方鲇生长激素完整cDNA的克隆及其DNA序列分析

基金项目: 

国家自然科学基金(项目号:39770591)

农业部重点实验室研究基金

第三世界科学院(意大利)研究基金(基金号:95-348RG/BIO/AS)资助

CLONING AND SEQUENCING OF FULL LENGTH cDNA IN SILURUS MERIDIONALIS GROWTH HORMONE

  • 摘要: 采用RT-PCR法和3'、5'RACE(Rapid amplification of cDNA ends)法从南方鲇脑垂体RNA克隆出生长激素(Growth hormone,GH)cDNA。此cDNA全长1087nt(含 Poly(A),其5'端非编码区长52nt、阅读框(Open reading frame,ORF)长603nt、3'端非编码区长415nt。其推导的GH由200个氨基酸组成,其中前24个氨基酸为信号肽部分。氨基酸序列比较表明,南方鲇GH与Pangasianodon gigas、Ictalurus punctatus和Heteropneustes fossilis三种鲇类的同源性分别为97.5%和95.0%,与鲢、鳙、草、鲤和鲫鱼的GH同源性达76.0%以上,而与人的GH(Ⅰ、Ⅱ)同源性最低为31.0%和29.5%.
    Abstract: The full length cDNA that encodes Siulurus meridionalis growth hormone(SmGH)was cloned from pituritary RNA with RT-PCR、3'and 5'RACE(rapid amplification of cDNA ends). The cDNA, about 1087nt long, had a coding sequence, 5'-and 3'-untranslated regions and a poly(A)chain. The cDNA could encode a prehormone of 200 amino acid(aa)including a putative signal peptide of 24aa. The primary structure of SmGH was compared with those of other 15 species growth hormones.
  • [1]

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出版历程
  • 收稿日期:  2001-03-04
  • 修回日期:  2001-11-05
  • 发布日期:  2002-05-24

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