注射LHRHa对泥鳅血清性类固醇激素变化研究
CHANGE DISCIPLINE OF SEX STEROID HORMONES IN SERUM OF LOACH (MISGURNUS ANGUILLICAUDATUS) INFECTED CHORIONIC�
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摘要: 研究了注射促黄体激素类似物(LHRHa)后,泥鳅(Misgurnus anguillicaudatus)血清性类固醇激素的变化规律,并探讨在泥鳅繁殖季节时,孕酮(P)、睾酮(T)和雌二醇(E2)对性腺发育的作用及调节机制。实验共分两组,对照组和实验组;对照组只注射生理盐水;实验组注射LHRHa,雌鱼0.2μg/g,雄鱼减半。注射前尾静脉采血,作为血液样本分析基础水平(0h),注射药物后分别在7、24、48、72和96h尾静脉采血。测定雌鱼睾酮、雌二醇和孕酮,雄鱼孕酮和睾酮血清浓度。实验结果表明:注射LHRHa,雌鱼血清睾酮和雌二醇浓度显著高于对照组,雄鱼血清睾酮和孕酮显著高于对照组(<0.05);24h浓度较高,雌鱼孕酮、睾酮和雌二醇分别为(0.710±0.082)ng/mL、(9.00±0.57)ng/mL和(696.4±26.2)pg/mL,雄鱼孕酮和睾酮分别为(0.527±0.121)ng/mL和(9.62±0.62)ng/mL。实验组雌鱼孕酮变化基本规律为,基础水平(0—7h)-逐渐升至最高(7—24h)-逐渐降至基础水平(24—48h)-维持基础水平(48—96h)。实验组雌鱼睾酮和雌二醇与雄鱼孕酮和睾酮变化规律基本相似,其规律为,逐渐上升至最高(0—24h)-逐渐降至基础水平(24—72h)-维持基础水(72—96h)。24h对照组雌鱼睾酮和雌二醇显著升高,浓度分别为:睾酮(2.20±0.18)ng/mL,雌二醇(269.1±36.6)pg/mL。对照组雄鱼血清孕酮和睾酮浓度实验期间均无显著变化。研究认为:LHRHa能够刺激泥鳅性类固醇激素分泌,特别是睾酮的分泌,显著提高雌鱼性腺指数(GSI)。但刺激P的分泌调控能力有限,实验期间处于较低水平,诱导排卵效果差,泥鳅的性类固醇激素可能有特殊的调节机制。雌二醇和睾酮对性腺成熟有重要作用,孕酮对介导卵细胞最终成熟和排卵可能起重要作用,而雌二醇和睾酮无明显效果。Abstract: This experiment was to clarify the change of sex steroid hormone in serum of loach(Misgurnus anguillicaudatus) infected LHRHa,and investigate actions of progesterone(P),testosterone(T) and estradiol(E2) to gonadal development and regulatory mechanism.It contented two groups,control group and test group.The fish of control group were only injected physiological saline,and the fish of test group were injected chorionic gonadotropin analogue [D-Ser(tBu)6,Pro9-NEt](LHRHa)(female fish 0.2ug/g and male fish 0.1ug/g).Blood samples were collected from caudal vein at 0,7,24,48,72 and 96h after implantation respectively,and T,E2 and P contents in serum were measured.These results showed that T and E2 in serum of female fish infected LHRHa were significantly higher than those of control group(P0.05).24h after infection LHRHa,P,T and E2 contents of female fish were 0.710±0.082ng/mL,9.00±0.57ng/mL and 696.4±26.2pg/mL respectively;P and T of male fish were 0.527±0.121ng/mL and 9.62±0.62ng/mL respectively.Change discipline of female fish in test group was as following rule,basis level(0—7h)-to rise gradually to the peak value(7—24h) to go down to basis level(24—48h) to maintain basis level(48—96h).The change discipline of T and E2 in female fish and P and T in male fish were similar,to rise gradually to the peak value(0—24h) to go down gradually to basis level(24—48h) to maintain basis level(48—96h).After 24h,the T and E2 contents of female fish in control group been significantly hoisted to 2.20±0.18ng/mL and 269.1±36.6pg /mL,but the P and T contents of male fish in control group changed unobviously(P0.05).The experimental results indicated that LHRHa could stimulate sex steroid hormone secretion of loach to female loach,especially T,and raised significantly the gonadosomatic index(GSI),but it had poor effect to induce ovulation.The reason may be that it had especial regulatory mechanism of steroid hormone.E2 and T in loach could promote sex gland maturity.P may play important role in final maturity of oocyte and ovulation in loach,and E2 and T had no obvious action in this way.
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Keywords:
- Loach /
- LHEHa /
- Progesterone /
- Testosterone /
- Estradiol
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[1] Zhao H H,Liu X C,Liufu Y Z.Seasonal cycles of ovarian development and serum sex steroid levels of female grouper Epinephelus Coioides[J].Acta Scientiarum Naturalium universitatis Sunyatsen,2003,42(6):56-63[赵会宏,刘晓春,刘付永忠.斜带石斑鱼雌鱼卵巢发育与血清性类同醇激素的生殖周期变化.中山大学学报,2003,42(6):56-63]
[2] Zhao W X.Testosterone and 11-oxotestosterone changes during an annual cycle in blunt snout beram,Megalobrama Amblycephala[J].Oceanologia Et Limnologia Sinica,1991,22 (5):412-416[赵维信.团头鲂两种雄性激素浓度的周年变化.海洋与湖沼,1991,22(5):412-416]
[3] Hou Y Y.Effects of gunadotropin releasing hormone (GnRH) and dopamine on gonadotropin (GTH) secretion from in vitro pituitary cells of rainbow trout[J].Jouranal of lake Sciences,2001,13(1):51-56[侯亚义.促性腺激素释放激素和多巴胺对虹鳟(Oncorhy-nchus mykiss)离体脑垂体细胞分泌促性腺激素的影响.湖泊科学,2001,13(i):51-56]
[4] King H R,Pankhurst N W.Effect of short-term temperature reduction on ovulation and LHRHa responsiveness in female Atlantic salmon(Salmo salar) maintained at elevated water temperatures[J].Aquaculture,2004,238:421-436
[5] Kusat A M..Gonadal oocyte development in LHRHa hormone treated European Sea Bass (Dicentrarchus labrax L,1758) Broodstock[J].Turk J Vet Anim Sci,2005,29:83-87
[6] Shein N L.Ovarian development and final oocyte maturation in cultured sevenband grouper Epinephelus septemfasciatus[J].Fisheries Science,2004,70:360-365
[7] Jalabert B.In vitro oocyte maturation and ovulation in rainbow trout (Salmo gairdneri) northern pike(Esox lucius),and goldfish (Carassius auratus)[J].J.Fish.Res.Board.Canada.,1976,33:974-988
[8] Crim L W.Control of gonadotrupic homone release in trout:influence of synthetie LH-RH and LH-RH analogues in viva and in vizre[J].Life Sci.,1981,28:129-155
[9] Qn W L,Pan W Z,Guo J X.Inductive effect of 17a-hydroxy20β-dihydroprogesterone on ovulation of loach,Misgurnus AnguiUigaudatus (Cant-or)[J].Journal of Fisheries of China,1990,14(2):165-169[曲维良,潘伟志,郭佳祥.17α-羟基-20β-双羟孕酮诱导泥鳅排卵的效应.水产学报,1990,14(2):165-169]
[10] Zhou D G,Zheng W M,Zhang D X.Changes of sex steroids in Mono-pterus Albus during its spawning induced with injection of LHRH-A[J].Acts Hydrobiologica Sinica,1995,19(4):311-316[周定刚,郑维明.张大祥.催产时黄鳝性类同醇激素含量变化的研究.水生生物学报,1995,19(4):311-316]
[11] Zhang L H,Zhang W M,Lin H R.The fcedback effects of 4-androstene-3,17or-dine and 17-methyl-testosterone on gonadal 1 development in the female Anguilla Japonica[J].Journal of Fishcries of China,2000,24(5):407-411[张利红,张为民,林浩然.雄烯二酮和甲基睾酮诱导雌性日本鳗细性腺发育的反馈调节作用.水产学报,2000,24(5):407-411]
[12] Lin H R,Zhang M L,Zhang S M.Effects of sex steroids on gona-dotropin synthesis and secretion,as well as ovarian development in female silver eel[J].Acts Hydrobiologica Sinlca,1994,18(3):272-279[林浩然,张梅丽,张素敏,等.鳗鲡繁殖生物学研究V.性类同醇激素诱导雌鳗促性腺激素(GtH)分泌和卵巢发育的作用.水生生物学报,1994,18(3):272-279]
[13] Zhang L H,Zhang W M,Lin H R.The mobilization of calcium and fat during the steroid hormone-induced maturation of female Japanese Eel (Anguilla japonica)[J].Acts Scientiarum Naturalium universitatis Sunyatsen,2001,40(92):86-88[张利红,张为民,林浩然,等.性类同醇激素诱导雌性日本鳗鲡性腺发育过程中钙和脂肪的动员.中山大学学报,2001,40(92):86-88]
[14] Tang X C,Liu C W.Effects of exagenous hormonas on serum sex seteroid hormones in Plectorhynchus cinctus[J].Marine Science (Experiment Technology),2003,27(5):5-7[唐啸尘,刘楚吾.外源激素对花尾胡椒鲷血清性类固醇激素的影响.海洋科学(实验与技术),2003,27(5):5-7]
[15] Wen H S,Song H X,Yang L T.Study on the effects of exogenous hormone on the plasma testosterone and estradiollevels in cultured Japanese Flounder[J].Acts Oceanologica Sinica,2006,28(4):115-120[温海深,宋海霞,杨立廷.外源激素对养殖牙鲆血浆睾酮和雌二醇含量的影响研究.海洋学报,2006,28(4):115-120]
[16] Shu H,Liu X C,Lin H R.Development,sex steroid hormone secretion and uhrastructure of gonedotropic cell in Epinephelus akaara[J].Zoological Research,2005,26(4):422-428[舒琥,刘晓春,林浩然.LHRH-A缓释剂对雌性赤点石斑鱼卵巢发育、性类同醇激素分泌及脑垂体GTH细胞超微结构的影响.动物学研究,2005,26(4):422-428]
[17] Shu H,Liu X C,Zhao H H.Mih production by male rod spotted grouper (Epinephelus akaara) after injection of LHRHa,17P or DOM,alone or in combination[J].Acts Scientiarum Naturalium universitatis Suuyauen.2004,43(6):42-44[舒琥,刘晓春,赵会宏.注射LHRHa、17-P及其他药物组合诱导赤点石斑鱼排精研究.中山大学学报(自然科学版),2004,43(6):42-44]
[18] Song P,Xiong Q M.The annual cycle of serum estradiol and teatoserone in spontaneous sex-reversing fish Monopterus abus[J].Journal of Wuhan University (Natural Science Edition),1993,2:115-120[宋平,雄全沫.黄鳝血清睾酮和雌二醇含量周年变化规律的研究.武汉大学学报(自然科学版),1993,2:115-120]
[19] Zhao W H,Hong W S,Zhang Q Y.Comparative studies on the levels of sex serum steroids and the levels of 17α-P and prostaglandins of the gonads in the feral and cultured Bostrichthys sinensis[J].Journal of Xiamen University (Natural Science),2005,44(Suppl.):195-199[赵卫红,洪万树,张其永.等.中华乌塘鳢野生和养殖群体血清中性类固醇激素以及件腺17α-P和前列腺素含量的比较研究.厦门大学学报(自然科学版),2005,44(增刊):195-199]
[20] Lahnsteiner F,Bcrger B,Kletzl M.Effect of 17β-estradiol on gamete quality and maturation in two salmonid species[J].Aquatic Toxicology,2006,79:124-131
[21] Wang D S,Lin H R,Pn D Y.Domperidonc-LHRH-A-Induced Ovulation of the Bagrld Catfish,Mytus Macropterus and Leiocassis longirostris[J].Zoological Research,1998,19(3):191-196[王德寿,林浩然,蒲德永.注射促黄体素释放激素类似物和地欧酮诱导大鳍和长吻排卵的研究.动物学研究,1998,19(3):191-196]
[22] Fang Y Q,Weng Y Z,Hu X X.Distribution of sex steroid hormones and their receptors in the gonads and nerous system of am phioxus (Branchiostoma belcheri)[J].Acts Zoologica Sinica,2001,47(4):398-403[方永强,翁幼竹,胡晓霞.性类同醇激素及其受体在文昌鱼性腺和神经系统中的分布.动物学报,2001,47(4):398-403]
[23] Cai S L,Yang C H.Effect of 17a-hydroxyprogesterone on in vitro culture of shrimp (Penaeus chinensis)[J].Arine Fisheries Research,2000,21(2):7-11[蔡生力,杨丛海.17α-羟孕酮对离体培养的对虾卵巢组织发育的促进作用.海洋水产研究,2000,21(2):7-11]
[24] Zhang M Y,Zhu Y R.The comparative study on the ovulation increased in Misgugnus anguiUicaapatus(Autor) by different dosage of reserpine,dopamine and LHRH-A[J].Fisheries Science,1998,17(3):10-13[张明宇,朱逸仁.不同剂量的利血平LHRH.A促进泥鳅排卵的比较研究.水产科学,1998,17(3):10-13]
[25] Wu W W,Gao X P.Effects of progesterone on the fertility ability of human sperm[J].Journal of Andrology,1996,10(2):71-74[吴无畏.高晓平.孕酮对人精子受精能力的影响.男性学杂志,1996,10(2):71-74]
[26] Bing X,Ru S G,Hiroko I.The identification of vitellogenin induction by 17β-estradiol in male Carassius auratus[J].Journal of Fisheries of China,2004,28(3):236-240[邴欣,汝少国,Isoda Hiroko.17β-雌二醇对雄性金鱼卵黄原蛋白的传导作用.水产学报,2004,28(3):236-240]
[27] Ning Q J,Li E Z,Zhai X H.The regulation of exogenous exogenous estradiol in transcriptional activity of regeneratiug hepatocytes in rats[J].Acts Anatomica Sinica,2004,35(4):413-416[宁黔冀,李恩中,翟心慧.外源性雌二醇对大鼠再生肝细胞转录活性的调节.解剖学报,2004,35(4):413-416]
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