养殖乌鳢类立克次体分离纯化的初步研究
PRIMARY ISOLATION AND PURIFICATION OF RICKETTSIA-LIKE ORGANISM (RLO) FROM THE TISSUES OF OPHIOCEPHALUS ARGUS
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摘要: 1989年,Fryer等从导致智利海水养殖Coho salmon(Oncorhynchus kisutch)90%死亡率的病鲑组织感染的鲑细胞系中首次分离得到一种类立克次体(Kickettsia like organism,RLO)并命名为Piscirickettsia salmonis gen.nov.,sp.nov.。近十年来,这种被日益重视的、专性细胞内寄生的RLO(细胞内的细菌),已被证实为导致鱼类严重传染性疾病的致病因子.2001-2002年,作者首次发现湖北省某养殖场淡水养殖乌鳢(Op hiocep halus argus C.)突发性大规模发病死亡系RLO感染引起。由于乌鳢细胞系的建立尚在进行之中,因此从病变组织直接进行RLO的分离、纯化对进一步开展病原体分类及快速、准确的诊断技术等分子生物学研究至关重要。
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Keywords:
- Rickettsia-like organism(RLO) /
- Isolation /
- Purification /
- Ophiocephalus argus
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[1] Fryer J L,Lannan C N,Garces L H,et al.Isolation of a rickettsiales-like organism from diseased coho salmon (Oncorhynchus kisutch)in Chile[J].Fish Pathol.1990,25:107-114[2] Mauel M J,Miller D L.Piscirickettsiosis and piscirickettsiosis-like infections in fish:a review[J].Vet Microbiol,2002,87:279-289[3] Guo Q L,Chen Y X,Han X P.Pathology of haemorrrhagic mouth-openingdisease of the eel.Anguilla Japonica[J].Acta Hydrobiol Sin, 1997,21:327-331[郭琼林,陈燕新,韩先朴.鳗鲡出血性开口病的病理学研究.水生生物学报,1997,21:327-331][4] Guo Q L,Lu Q Z.Histopathological studies on the Edwardsielliasis of the eel [J].Acta Hydrobiol Sin, 1995,19:56-60[郭琼林,卢全章.鳗鲡爱德华氏菌病的组织病理学研究.水生生物学报,1995,19:56-60][5] Guo Q L,Jia W Z,Han X P,et al.Rickettsia-like organism infection in a freshwater cultured fish Ophiocephalus argus C.in China[J].Prog Nat Sci 2004,14[6] Zhang Q Z,Wu X Z,Pan J P.Purification and morphology of rickettsia-like organism (RLO) from the tissues of Crassostrea ariakensis Gould[J].High Technol Letters,2002,7:90-92[张其中,吴信忠,潘金培.近江牡蛎类立克次体的分离纯化及形态特点.高技术通讯,2002,7:90-92][7] Kuzyk M A,Thorton J C,Kay W W.Antigenic characterization of the salmonid pathgen Piscirickettsia salmonis[J].Infect Immun,1996,64:5205-5210[8] Barnes M N,Landolt M L,Powell D B,et al.Purification of Piscirickettsia salmonis and partial characterization of antigens [J].Dis Aquat Org,1998,33:33-41[9] Yuksel S A,Thompson K D,Ellis A E,et al.Purification of Piscirickettsia salmonis and associated phage particles [J].Dis Aquat Org,2001,44:231-235[10] Jamett A,Aguayo J,Miquel A,et al.Characteristics of monoclonal antibodies against Piscirickettsia salmonis[J].J Fish Dis,2001,24:205-215 Fryer J L,Lannan C N,Garces L H,et al.Isolation of a rickettsiales-like organism from diseased coho salmon (Oncorhynchus kisutch)in Chile[J].Fish Pathol.1990,25:107-114[2] Mauel M J,Miller D L.Piscirickettsiosis and piscirickettsiosis-like infections in fish:a review[J].Vet Microbiol,2002,87:279-289[3] Guo Q L,Chen Y X,Han X P.Pathology of haemorrrhagic mouth-openingdisease of the eel.Anguilla Japonica[J].Acta Hydrobiol Sin, 1997,21:327-331[郭琼林,陈燕新,韩先朴.鳗鲡出血性开口病的病理学研究.水生生物学报,1997,21:327-331][4] Guo Q L,Lu Q Z.Histopathological studies on the Edwardsielliasis of the eel [J].Acta Hydrobiol Sin, 1995,19:56-60[郭琼林,卢全章.鳗鲡爱德华氏菌病的组织病理学研究.水生生物学报,1995,19:56-60][5] Guo Q L,Jia W Z,Han X P,et al.Rickettsia-like organism infection in a freshwater cultured fish Ophiocephalus argus C.in China[J].Prog Nat Sci 2004,14[6] Zhang Q Z,Wu X Z,Pan J P.Purification and morphology of rickettsia-like organism (RLO) from the tissues of Crassostrea ariakensis Gould[J].High Technol Letters,2002,7:90-92[张其中,吴信忠,潘金培.近江牡蛎类立克次体的分离纯化及形态特点.高技术通讯,2002,7:90-92][7] Kuzyk M A,Thorton J C,Kay W W.Antigenic characterization of the salmonid pathgen Piscirickettsia salmonis[J].Infect Immun,1996,64:5205-5210[8] Barnes M N,Landolt M L,Powell D B,et al.Purification of Piscirickettsia salmonis and partial characterization of antigens [J].Dis Aquat Org,1998,33:33-41[9] Yuksel S A,Thompson K D,Ellis A E,et al.Purification of Piscirickettsia salmonis and associated phage particles [J].Dis Aquat Org,2001,44:231-235[10] Jamett A,Aguayo J,Miquel A,et al.Characteristics of monoclonal antibodies against Piscirickettsia salmonis[J].J Fish Dis,2001,24:205-215
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