鳜鱼外周血细胞显微和亚显微结构的观察

MICRO AND ULTRA-STRUCTURE OF PERIPHERAL BLOOD CELLS OF THE MANDARIN FISH, SINIPERCA CHUATSI (BASILEWSKY)

  • 摘要: 本文报道了鳜鱼外周血细胞的显微和亚显微结构。血涂片经过染色,可鉴别出红细胞、血栓细胞、淋巴细胞、单核细胞和嗜中性粒细胞;还见到幼稚的和正在分裂的红细胞,提示红细胞亦可在外周血液中通过直接分裂产生。白细胞中,血栓细胞体积最小,嗜中性粒细胞体积最大;单核细胞数目最少,血栓细胞数目最多。电镜下,红细胞中可见线粒体和高尔基复合体;淋巴细胞线粒体中可见类似髓样体的板层状结构;血栓细胞和单核细胞与其它鱼类的基本相似;鳜鱼的粒细胞,根据胞质内颗粒及其它细胞器的结构,将其分为Ⅰ型和Ⅱ型粒细胞,后者类似于其它鱼类的嗜中性粒细胞,前者属于哪种类型尚待进一步研究。此外,鳜鱼血液中还观察到发育不充分的巨噬细胞和类似浆细胞的细胞。

     

    Abstract: The peripheral blood cells of the mandarin fish,Siniperca chuatsi (Basilewsky) are morphologically described. On the stained smears five major cell types were recognized: erythrocyte,thrombocyte,lymphocyte,monocyte and neutrophil. Among these cells,thrombocyte is the smallest while neutrophil the biggest; and monocyte is the least encountered leucocyte,while thrombocyte the most abundant Mitochondria and Golgi complex were found in erythrocyte and structure similar to myeloid body in mitochondria of lymphocyte. The granulocytes were further classified into two types: type Ⅰ and type Ⅱ granulocytes based on the structures of intratytoplasmic granules and other organelles. The type Ⅱ granulocyte is similar to the neutrophil of other fish,but further stUdies are required to determine the type Ⅰgranulocyte. The observations of immature and dividing erythrocytes in the blood indicate that Peripheral erythrocytes may develop directly in the cireulation. In addition,developing macrophages and cells with chanaterishcs of plasma cells were found in the blood.

     

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