黄鳝性别的自然反转现象与血清蛋白关系的初步研究

RELATIONSHIP BETWEEN SEX REVERSAL AND SERUM PROTEINS IN MONOPTERUS

  • 摘要: 实验采用聚丙烯酰胺凝胶电泳方法,对49条黄鳝的血清蛋白进行了分析,实验结果表明雌、雄黄鳝随着性腺的发育,血清蛋白组分有所增加,雌鱼性腺Ⅲ—Ⅳ期为10—11条区带。雄鱼性腺Ⅰ到Ⅳ期为11—14条区带。性别反转的黄鳝血清蛋白的区带数明显增加,分析结果为20条区带,经CS-910薄层色谱扫描具有较多的吸收峰,极为有趣的是性别反转的黄鳝血清蛋白在凝胶负极一端的大分子脂蛋白和中等分子的球蛋白组分发生了很大的变化。而正极分子较小的白蛋白组分仍保持着雌鱼血清蛋白的吸收峰,因此作者认为黄鳝性别反转作用可能与血清蛋白中较大分子组分的增加有关。

     

    Abstract: Whole sera of sex-reversing individuals as well as of females and males in Monopterus were analysed respectively through polyacrylamide gel (7%, pH 8.8) disc electrophoresis. Resuits show that the electrophoretic bands of serum proteins in both sexes of Monopterus increase with gonad development. The electrophoretic patterns obtained by 7% gel showed 10—11 serum protein bands in female Monopterus (gonadal stage Ⅲ-Ⅳ); while those in male were 11—14 (gonadal stages Ⅰ-Ⅳ). However the number of serum proteins of sex-reversing Monopterus has even more distinct increase, serum protein showing more than 20 bands in the electrophoreric gel. The serum albumin of inter sexual Monopterus had 4 bands in the electrophoretic gel, being the same as that of the female. The number of electrophoretic bands of serum liporoteins in females, located the negative pole, was identical with that of the male individuals, having 5 bands. In intersexual fish the r-globulin and B-globulin proteins of medial molecular size were distinctly different from those of the female and the male individuals, the paper showing 11—12 bands in the former.For this reason, the authors consider that the increase of the larger molecular sized serum globulins is somewhat correlated to the sex-reversing process in Monopterus.

     

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