荧光物质浸泡标记稀有鮈鲫和彭泽鲫仔、稚鱼

USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR

摘要: 研究了茜素络合物和盐酸四环素对稀有鮈鲫和彭泽鲫仔、稚鱼进行浸泡标记的方法和效果。茜素络合物在浸泡浓度50-100mg/L,浸泡时间持续6-24h的范围内,对两种鱼都有很好的标记效果;盐酸四环素在浸泡浓度150-250mg/L,浸泡时间12-24h的范围内,对彭泽鲫有较好的标记效果,但对稀有鲫有极高的致死率。在荧光显微镜下观察,茜素络合物呈橘红色、盐酸四环素标记区呈金黄色荧光反应。

Abstract: The present paper deals with methods and effects of using alizarincomplexone (ALC)and chlorydrate of tetracycline (CHTC) to mark larval and juvenile Gobiocypris rarus and Carassius auratus pengze Var. To immerse the two species in 50-100mg/ L ALC solutions for 6-24h, a well-defined mark on otolith was induced.A well-defined mark on otolith was also observed when immersing larval Carassiusauratus pengze Var in 150-250mg/L CHTC solutions for 12-24h. However, onlyimmersing -caused mortality was observed in G. rarus when immersed in CHTC solution. suggesting that TC may not be suitable for being used as marking solution.Under UV light,the mark of ALC in the otoliths is a scarlet-pink fluorescent zone,and that of CHTC is a yellow fluorescent zone.