Abstract: Colchicine and cytochalasin B were used to investigate their influence on the conjugation of Tetrahymena thermophila, especially at the late stage. In the experimental group that was treated with colchicine, the percent of 34KD、37KD、46KD and 57KD cortical cytoskeletal proteins changed a lot, while in the experimental group treated with cytochalasin B, the percent of 40KD and 74KD proteins changed numeraely. According to some relevant papers, we inferred that 34KD、37KD、46KD and 57KD may be tubulin, while 40KD and 74KD may be microfilamental proteins, furthermore, these proteins are all important to the morphogenesis of Tetrahymena thermophila during conjugation, and it needs further investigation.