Abstract: The xenobiotics in water absorbted by fish can be rapidly transported to the gall bladder.The bioconcentration factor(BCF)of PCP in gall bladder of hybrid crucian carp was 11365after 48h exposure.The accumulation and excretion of PCP in gall bladder were increasedwith the rise of water temperature.More than 90% of the whole PCP were conjugated-PCP in the bile of the grass carp.The binary concentration of the conjugated-PCP increased withthe exposure duration and reached a level of 904 mg/kg and a BCF of 6027 at 48h. The bileof grass carp exposed in both tap water and Donghu lake water for 48h was founded 24 and25 compounds(chromatogram)respectively as measured by GC.The results suggest that it issuitable to monitor xenobiotics in water.