两株钝顶螺旋藻紫外诱变株的特征
CHARACTERISTICS OF TWO SPIRULINA PLATENSIS MUTANTS INDUCED BY ULTRAVIOLET
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摘要: 采用紫外诱变的方法筛选获得了两株优良的稳定钝顶螺旋藻突变株M1-3和M5-1.与出发株相比,M5-1较粗大,M1-3较细,但很长,藻体螺旋数超过40;两株突变株的生长速度和光合放氧速率均有显着提高;M1-3的藻蓝蛋白含量高于出发藻株202%;突变株的长碳连不饱和脂肪酸含量高于出发藻株,总脂中M1-3含花生四烯酸(20∶4)4.93%、M5-1含EPA(20∶5)2.49%.两株突变株对NH4+和Zn2+的抗性也发生了改变.Abstract: Spirulina platensis was induced by ultraviolet irradiation as a mutagen. Two good mutants,M1-3 and M5-1,were selected. M5-1 was bigger than PS(Paprent Strain),and M1-3 was thinner and much longer with its spiral number of one filament being over 40;The growth and photosynthetic O2 evolution rates of two mutants were higher than those of PS obviously. Phycocyanin content in M1-3 was 20.2% higher than those of PS. The two mutants had more long carbon chain polyunsaturated fatty acids. The M1-3 contained 4.93% arachidonic acid(20∶4),and M5-1 contained 2.49% EPA(20∶5)in the total fat. The antitoxic abilities of two mutants to toxic ions,NH4+ and Zn2+ were different from PS also.
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