异源四倍体鲫鲤雌雄差异的RAPD标记
RAPD MARKER OF THE DIFFERENCE BETWEEN THE MALE AND FEMALE OF ALLOTETRAPLOID CRUCIAN-CARP
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摘要: 异源四倍体鲫鲤是从红鲫和湘江野鲤的杂交后代选育出来的,已经形成了一个遗传性状稳定的四倍体鱼新种群。用异源四倍体鲫鲤(雄性)和二倍体白鲫(雌性)生产的三倍体湘云鲫已经在全国推广应用。因此,如果能够了解异源四倍体鲫鲤的性别分化机制,人为地控制异源四倍体鲫鲤的性别分化,生产出大量的超雄鱼,这对于三倍体湘云鲫的产业化生产有重要的意义。刘少军等对异源四倍体鲫鲤的染色体组型进行了分析,并没有发现异源四倍体鲫鲤有明显的特化的性染色体,这说明通过细胞遗传学研究异源四倍体鲫鲤的性别遗传机制是有困难的。
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Keywords:
- Allotetraploid crucian-carp /
- RAPD /
- Sex-linked marker
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F.Genetics and engineering[M].Shanghai: Scientific and Technology Publisher,1999.[吴清江,桂建芳.鱼类遗传育种工程.上海:上海科学技术出版社,1999][17] Lou Y D.Fish breeding[M].Beijing: China agricultural publisher,1999.[楼允东.鱼类鱼种学.北京:农业出版社,1999][18] Liu Y.Propagation physiology of main cultivated fish in China[M].Beijing: Agricultural publishing house,1999.[刘筠.中国养殖鱼类繁殖生理学.北京:农业出版社,1999] Liu Y,Zhou G J.Cytological study on the gonadal development of F1:hybrid produce by crossing Carassius auratus L.(♀)with Cyprinus carpio(♂)[J].Acta Hydrobiol Sin,1986,10(2):101-107[刘筠,周工健.红鲫(♀)×湘江野鲤(♂)杂交一代生殖腺的细胞学研究.水生生物学报,1986,10(2):101-107][2] Liu S J,Cao Y C,He X X,et al.The formation of tetraploid hybrids of common carp with red crucian carp and the evolutionary significance of tetraploidization in vertebrate[J].Engineering science,2001,3(12):33-41[刘少军,曹运长,何晓晓,等.异源四倍体鲫鲤群体的形成及四倍体化在脊椎动物中的作用.中国工程科学,2001,3(12):33-41][3] Liu S I,Liu Y,Zhou G J,et al .The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization[J].Aquaculture,2001,192: 171-186[4] Li J Z,Zhang X J,Liu S J,et al.Studies on the gonadal development in allotetraploi hybrids Carassius auratus L.(♀)with Cyprinus carpio(♂)[J].Acta Hydrobiol Sin,2002,26(2):116-122[李建中,张轩杰,刘少军,等.异源四倍体鲫鲤的性腺发育[J].水生生物学报,2002,26(2):116-122][5] LI J Z,Lu S Q,Liu S J,et al.RAPD analysis of genetic variation between the allotetraploid hybrid of red crucian carp×common carp and their original parents[J].J Fish China,2002,27(5):403-408 [李建中,鲁双庆,刘少军,等.异源四倍体鲫鲤及其原始亲本遗传变异的RAPD分析[J].水产学报,2002,27(5):403-408][6] Liu S J,Sun Y D,Zhang C,et al.Production of gynogenetic progeny from allotetraploid hybrids red crucian carp×common carp[J].Aquaculture,2004,236:193-200[7] Chang Z J,Du Q Y.Sex determination and sex chromosome in fishes[J].Freshwater Fisheries,2002,32(2):56-58.[常重杰,杜启艳.鱼类的性别决定和性染色体.淡水渔业,2002,32(2):56-58][8] Zhou L,Wang Y,Gui J F.Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp(Carassius auratus gibelio Bloch)as revealed by RAPD assays[J].J.Mol Evol,2000,51:498-506[9] Balazs Kovacs,Sandor E,Richard B,et al.Male-specific DNA markers from African catfish(Clarias gariepinus)[J].Genetica,2001,110:267-276[10] Mcgow AN C.And Davidson W.S.The RAPD technique fails to detect a male-specific genetic marker in Atlantic Salmon[J].Journal of Fish Biology,1998,53:1134-1136[11] Iturra P,Medrano J F,Bagley M,et al.Identification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout [J].Genetica,1998,101:209-213[12] Bello N,Sanchez A.The identification of a sex-specific DNA marker in the ostrich using a random amplified polymorphic DNA(RAPD)assay[J].Molecular Ecology,1999,8:667-669[13] Qiu T,Lu R H,Xiang C M,et al.Indetification of sex differentiation in Eriocheir sinensis with RAPD[J].J Fish China,1998,22(2):175-177.[邱涛,陆仁后,项超美,等.用RAPD技术识别中华绒螯蟹性别差异.水产学报,1998,22(2):175-177][14] Chang Z J,Zhou R X,Yu Q X.Genetic variation of two loach species revealed by RAPD analysis[J].Acta Zoo .Sin,2001,47(1):89-93.[常重杰,周荣家,余其兴.两种泥鳅不同群体遗传变异的RAPD分析.动物学报,2001,47(1):89-93][15] Song P,Pan Y F,Xiang Z et al.RAPD markers and genetic diversity in Pelteobagrus Fulvidraco[J].J Wuhan Univ.(Nat.Sci.Ed.),2001,47(2):233-237.[宋平,潘云峰,向筑,等.黄颡鱼RAPD标记及其遗传多样性的初步分析.武汉大学学报,2001,47(2):233-237][16] Wu Q J,Gui J F.Genetics and engineering[M].Shanghai: Scientific and Technology Publisher,1999.[吴清江,桂建芳.鱼类遗传育种工程.上海:上海科学技术出版社,1999][17] Lou Y D.Fish breeding[M].Beijing: China agricultural publisher,1999.[楼允东.鱼类鱼种学.北京:农业出版社,1999][18] Liu Y.Propagation physiology of main cultivated fish in China[M].Beijing: Agricultural publishing house,1999.[刘筠.中国养殖鱼类繁殖生理学.北京:农业出版社,1999]
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