满江红鱼腥藻glnA启动区的克隆及在烟草中的活性表达
THE CLONE OF ANABAENA AZOLLAE GLNA PROMOTER AND ACTIVITY EXPRESSION IN TRANSGENIC TOBACCO
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摘要: 利用PCR技术获得满江红鱼腥藻glnA启动区,经克隆测序后构成谷氨酰胺合成酶基因启动子驱动的GUS基因表达载体.用基因枪转化,将表达载体导入烟草中,在其叶片和茎中检测到GUS活性,而在烟草根中未见表达.实验结果对真核生物与原核生物间基因表达调控、转录因子识别的研究以及构建实用载体具有一定价值.Abstract: The promoter of Anabaena aazollae glnA gene was obtained by PCR procedure.A binary vector containing the chimeric gene of glnA / GUS was constructed after cloning and sequencing.The expression vector was transformed into tobacco by microprojectile bombardment.The GUS activities were examined histochemically in leaves and stems of the transgenic tobacco, but not in its roots.The result was valuable both in the regulation of gene expression, the recognition of transcriptional factors between eukaryota and prokaryota and the construction of practical vectors.
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Keywords:
- glnA promoter /
- Activity expression /
- Anabaena azollae /
- Tobacco
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[1] C Peter Wolk, Avigad Vonshak, Patricia Kehoe et al. construction of shuttle vector capable of conjugativetransfer from E. Coli to nitrogen-fixing filamentuous cyanobacteria [J]. Proc Natl Acad Sci US4, 1984, 81:1561-1565[2] Sambrook J, et al. Molecular Cloning: A laboratory Manunal, 2nd, cold spring Harbor. [M]. New York,1989.[3] John C Sanford, Theodore M Klein, Edward D Wolf, et al. Delivery of Substances into cells and Tissue Using a Particle Bombardment Process [J]. Particulate Sci Technol, 1987, 5:27-37[4] Richard A, Jefferson Kate J. Wilson, plant Molecular Biology Manual [M]. 1991, B14: 1-33.[5] 印莉萍,刘祥林,林忠平,植物谷氨酰胺合成酶基因以及基因表达[J],生物工程进展,1995,15(2):36-41[6] 秦松,张健,李文斌等,用基因枪将GUS基因导入褐藻细胞中表达[J].海洋与湖沼,1994,25(4):353-356[7] Wang S. J., Li H., Research of direct gene transfer in the cell of porphyra haitanensis chang et zheng [A].(5 th) International phycological congress [C], Qing Dao, China 1994, 250 C Peter Wolk, Avigad Vonshak, Patricia Kehoe et al. construction of shuttle vector capable of conjugativetransfer from E. Coli to nitrogen-fixing filamentuous cyanobacteria [J]. Proc Natl Acad Sci US4, 1984, 81:1561-1565[2] Sambrook J, et al. Molecular Cloning: A laboratory Manunal, 2nd, cold spring Harbor. [M]. New York,1989.[3] John C Sanford, Theodore M Klein, Edward D Wolf, et al. Delivery of Substances into cells and Tissue Using a Particle Bombardment Process [J]. Particulate Sci Technol, 1987, 5:27-37[4] Richard A, Jefferson Kate J. Wilson, plant Molecular Biology Manual [M]. 1991, B14: 1-33.[5] 印莉萍,刘祥林,林忠平,植物谷氨酰胺合成酶基因以及基因表达[J],生物工程进展,1995,15(2):36-41[6] 秦松,张健,李文斌等,用基因枪将GUS基因导入褐藻细胞中表达[J].海洋与湖沼,1994,25(4):353-356[7] Wang S. J., Li H., Research of direct gene transfer in the cell of porphyra haitanensis chang et zheng [A].(5 th) International phycological congress [C], Qing Dao, China 1994, 250
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