三种免疫制剂对真鲷弧菌病的免疫保护性
THE IMMUNE PROTECTIVE EFFECTS OF THREE KINDS OF VACCINATION AGAINST VIBRIOSIS IN MARINE-CAGE CULTURED RED SEA BREAM(PAGRUS MAJOR)
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摘要: 菌体疫苗按不同的方式对真鲷进行免疫2周后,对实验鱼均具有免疫保护性,免疫保护性最好的免疫组,免疫保护率在初次免疫后高达60%,强化免疫后免疫保护率可提高到80%;粗制LPS经去毒处理后初次免疫真鲷,不同浓度的LPS对实验鱼具有不同程度的免疫保护性,强化免疫后,免疫保护率均有明显的提高,浓度越高,免疫保护性越强,对真鲷的免疫保护率最高可达90%;最小弧菌产生的外毒素经福尔马林灭活后制成毒素苗,这种毒素苗能产生较好的免疫保护性,其免疫保护率可达80%,这表明外毒素不仅是最小弧菌产生的毒力因子,同时也是菌体产生的有效保护性抗原.Abstract: Red sea bream(Pagrus major)were injected intramuscularly and direct immersion with Vibrio mimicus formalin killed cells)FKC), crude lipopolysaccharide(LPS)preparations and formalinized extracellular toxin(toxoid)of V. mimicus , respectively. The crude LPS prepared by the phenol water method had a little toxicity. The crude LPS was being heated to reduce its toxicity. The effectiveness of vaccination against Vibrio mimicus were evaluated two weeks after the immunization. The results showed that good protections were induced in red sea bream by FKC, LPS and toxoid. After booster immunization, the degrees of protection were enhanced.
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[1] 吴后波,潘金培.海水网箱养殖真鲷弧菌病病原生物学[C]. 迈向21世纪的渔业科技创新. 北京:海洋出版社,2000, 672-677[2] Whistiler R L. Methods in carbohydrate chemistry Vol V, New York: Academis Pdress, 1965, 83-91[3] Dubois M, Furer E, Sandoff J.C. et al. Colorimetric methods for th e determination of sugars and related substances [J].Anal Chem, 1956,28(2): 350-356[4] 吴后波,潘金培.海水养殖真鲷弧菌病病原菌外毒素的分离、纯化及生物学活性[J]. 海洋与湖沼(已接受)[5] Baxa D V, Kawai K, Kusuda R. In vitro and in vivo activities of Flexibacter maritimus toxins[J].Rep. Usa Mar. Bio. Inst., Kochi Univ., 198 8, 10:1-8[6] Hastings T S, Ellis A E. Detection of antibodies induced in rainbow trout by different Aeromonas salmonicida vaccie preparations[J]. Journa l of Aquatic Animal Health, 1990, 2:135-140[7] Lund V, Jorgensen T, et al. Humoral immune response in Atlantic Sal mon,Salmo Salar, to cellular and extracellular antigens of Aeromonas salmo nicida[J].Journal of Fish Disease, 1991 14:443-452[8] Arnesen J A, Bjornsdottir R, et al. Immunological responses in Atla ntic Salmon, Salmo Salar L, against purified serine protease and haemolysins from Aeromonas salmonicida[J]. Journal of Fish Disease, 1993, 16:409-423[9] 陈昌福.草鱼对鱼害粘球菌类脂多糖的免疫反应[J].淡水渔业,1989, 4:3-5 吴后波,潘金培.海水网箱养殖真鲷弧菌病病原生物学[C]. 迈向21世纪的渔业科技创新. 北京:海洋出版社,2000, 672-677[2] Whistiler R L. Methods in carbohydrate chemistry Vol V, New York: Academis Pdress, 1965, 83-91[3] Dubois M, Furer E, Sandoff J.C. et al. Colorimetric methods for th e determination of sugars and related substances [J].Anal Chem, 1956,28(2): 350-356[4] 吴后波,潘金培.海水养殖真鲷弧菌病病原菌外毒素的分离、纯化及生物学活性[J]. 海洋与湖沼(已接受)[5] Baxa D V, Kawai K, Kusuda R. In vitro and in vivo activities of Flexibacter maritimus toxins[J].Rep. Usa Mar. Bio. Inst., Kochi Univ., 198 8, 10:1-8[6] Hastings T S, Ellis A E. Detection of antibodies induced in rainbow trout by different Aeromonas salmonicida vaccie preparations[J]. Journa l of Aquatic Animal Health, 1990, 2:135-140[7] Lund V, Jorgensen T, et al. Humoral immune response in Atlantic Sal mon,Salmo Salar, to cellular and extracellular antigens of Aeromonas salmo nicida[J].Journal of Fish Disease, 1991 14:443-452[8] Arnesen J A, Bjornsdottir R, et al. Immunological responses in Atla ntic Salmon, Salmo Salar L, against purified serine protease and haemolysins from Aeromonas salmonicida[J]. Journal of Fish Disease, 1993, 16:409-423[9] 陈昌福.草鱼对鱼害粘球菌类脂多糖的免疫反应[J].淡水渔业,1989, 4:3-5
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