酪蛋白小肽对幼龄草鱼生长和饲料利用的影响
EFFECT OF SMALL PEPTIDES FROM CASEIN ON GROWTH AND FEED UTILIZATION IN JUVENILE GRASS CARP CTENOPHARYNGODON IDELLA
-
摘要: 本实验为酪蛋白小肽在幼龄草鱼营养生理上的意义研究.225尾(3.76±0.16g)草鱼分为3组,每组75尾鱼,每25尾鱼饲于0.25m3的水族箱中,每组设3个平行水族箱.对照组添加0.5%酪蛋白,试验组1、2日粮中分别添加0.5%酶解酪蛋白(80%以上为小肽,平均链长2.98),0.5%酸解酪蛋白(80%以上为游离氨基酸).试验期6d,水温为27.6±3.1℃.结果表明:添加0.5%酶解酪蛋白组草鱼相对生长率、蛋白效率比、饲料效率比和血浆中镁含量与小肽总量均显著高于添加0.5%酪蛋白组和10.5%酸解酪蛋白组(P<0.05).草鱼日粮中添加一定比例的小肽可提高饲料表观消化率和蛋白消化率,增加血液循环中生物活性肽的含量,减少肝胰脏和肠系膜脂肪储积,提高机体对日粮中氨基酸的利用率,从而增加体内氮沉积,提高生长速度.Abstract: grass carps of 3.74?.23g were used in an experiment,the effect of small peptides on growth performance of grass carp Ctenopharyngodon idella.The fish was divided into three diet groups and reared in 3 triplicate aquarium(0.25m3) with 25 fishes in each aquarium for 56 days.The diets of test group 1,2 and control group included in 0.5% enzymatic casein(small peptides and average length of peptides 2.98),0.5% acidolysic casein(free amino acids)and 0.5%casein.The result showed that the relative growth rates(RGR),feed conversion ratio(FCR),protein efficiency ratio(PER),contents of magnesium and small peptides in blood plasma on fish in 0.5% enzymatic casein group were significantly higher than those in 0.5% casein group and 0.5% acidolysic casein group (p<0.05).These results indicated that small peptides could improve the ratio of apparent digestion and protein digestion and increase the total amount of small peptides in blood circulation and the retention of nitrogen in body,accordingly reducing the store of lipid in hepatopancreas and mesentery,and to advance the utilization of protein in diet.
-
Keywords:
- Ctenopharyngodon idella /
- Small peptides
-
-
[1] Takeda M,Takii K.Gustation and nutrition in fishes:application to aquaculture[M].London:Chapman Hall,1992,271-287[2] Erba D,Ciappellano S,Testolin G.Effect of caseinphosphopeptides on inhibition of calcium intestinal absorption due to phoshate[J].Nutrition Research,2001,(21):649-656[3] Xue M,Xie S Q,et al.Advances in studies on fish feeding stimulants[J].Acta Hydrobiologia Sinica,2003,27(6):639-643.[薛敏,解绶启,崔奕波,等.鱼类促摄食物质研究进展.水生生物学报,2003,27(6):639-643][4] Parisini P,Scicipioni P.Effects of peptide compents in a proteolysate in piglet nutrition [J].Zootecnica e Nutriz-ione-animale.1989,(15):637-644[5] Huang F,Yan A S,Zhang G R,et al.Effect of different dietary protein content on activities of pepsin and amylase of silurus meridionalis chen[J].Acta Hydrobiologia Sinica,2003,27(5):451-456[黄峰,严安生,张桂蓉,等.不同蛋白含量饲料对南方鲇胃蛋白酶和淀粉酶活性的影响水生生物学报,2003,27(5):451-456][6] Gilderg A.,Stenberg E A new process for advanced utilization of shrimp waste[J].Process Biochem.2001,36:809-812[7] Buchmann.N.B,Boza J Protein V.enzymic protein hydrolysates:Nitrogen utilization in starved rats[J].J.Agric.Food Chem.1979,30:583-589[8] Leonard A,Maynard A.B.Animal nutrition[M].New York:McGraw-Hill Book Company,2000.236-249[9] Infante J L Z Nutritional rehabilation of malnaurished rats by di-and tripeptides:nitrigon metabolism and intestinal response[J].J.Nutr.Biochem.,1992,(3):285-290[10] Tian LX,Lin D.Changes in digestive enzyme activities in grass carp Ctenopharyngodon idellus after feeding of two test diets[J].Acta Hydrobiologia Sinica,1993,17(1):58-65[田丽霞、林鼎,草鱼摄食两种蛋白质饲料后消化酶活性变动比较.水生生物学报,1993,17(1):58-65][11] Daneil H,Baumann SA.Physiological importance and characteristics of peptide transport in intestinal epithelial cells[M].New York:EAAP Publication,1994[12] Maria K.K.,Bamba T.Iron speciation in intestinal contents of rats fed meal composed of meat and non meat sources of protein and fat[J].Food Chem.1995,52:47-56 Takeda M,Takii K.Gustation and nutrition in fishes:application to aquaculture[M].London:Chapman Hall,1992,271-287[2] Erba D,Ciappellano S,Testolin G.Effect of caseinphosphopeptides on inhibition of calcium intestinal absorption due to phoshate[J].Nutrition Research,2001,(21):649-656[3] Xue M,Xie S Q,et al.Advances in studies on fish feeding stimulants[J].Acta Hydrobiologia Sinica,2003,27(6):639-643.[薛敏,解绶启,崔奕波,等.鱼类促摄食物质研究进展.水生生物学报,2003,27(6):639-643][4] Parisini P,Scicipioni P.Effects of peptide compents in a proteolysate in piglet nutrition [J].Zootecnica e Nutriz-ione-animale.1989,(15):637-644[5] Huang F,Yan A S,Zhang G R,et al.Effect of different dietary protein content on activities of pepsin and amylase of silurus meridionalis chen[J].Acta Hydrobiologia Sinica,2003,27(5):451-456[黄峰,严安生,张桂蓉,等.不同蛋白含量饲料对南方鲇胃蛋白酶和淀粉酶活性的影响水生生物学报,2003,27(5):451-456][6] Gilderg A.,Stenberg E A new process for advanced utilization of shrimp waste[J].Process Biochem.2001,36:809-812[7] Buchmann.N.B,Boza J Protein V.enzymic protein hydrolysates:Nitrogen utilization in starved rats[J].J.Agric.Food Chem.1979,30:583-589[8] Leonard A,Maynard A.B.Animal nutrition[M].New York:McGraw-Hill Book Company,2000.236-249[9] Infante J L Z Nutritional rehabilation of malnaurished rats by di-and tripeptides:nitrigon metabolism and intestinal response[J].J.Nutr.Biochem.,1992,(3):285-290[10] Tian LX,Lin D.Changes in digestive enzyme activities in grass carp Ctenopharyngodon idellus after feeding of two test diets[J].Acta Hydrobiologia Sinica,1993,17(1):58-65[田丽霞、林鼎,草鱼摄食两种蛋白质饲料后消化酶活性变动比较.水生生物学报,1993,17(1):58-65][11] Daneil H,Baumann SA.Physiological importance and characteristics of peptide transport in intestinal epithelial cells[M].New York:EAAP Publication,1994[12] Maria K.K.,Bamba T.Iron speciation in intestinal contents of rats fed meal composed of meat and non meat sources of protein and fat[J].Food Chem.1995,52:47-56
计量
- 文章访问数: 853
- HTML全文浏览量: 0
- PDF下载量: 554