THE IDENTIFICATION OF NEW THALASSIOSIRA FLABELLATA SP. NOV., WITHIN THE GENUS THALASSIOSIRA CLEVE FROM DAYA BAY OF THE SOUTH CHINA SEA
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摘要: 为澄清广东大亚湾水域海链藻属的物种多样性, 研究建立了它的单克隆培养株系。结合光镜和扫描电镜下的形态学特征, 以及基于核糖体大、小亚基序列的分子系统发育分析, 报道了中国海链藻属的1个新种, 即扇形海链藻Thalassiosira flabellata sp. nov. X.H. Guo, Y.Q. Guo & Y. Li。该种以短链状群体或单细胞生活, 壳面具有一圈壳缘支持突、一个中央支持突和一个壳缘唇形突。壳面孔纹束状排列, 是此种的典型特征。此种与帕尔海链藻T. partheneia较为相似, 区别特征如下: 此种的壳面支持突和唇形突均具有外管, 而帕尔海链藻的突起没有外管, 只有内管; 其次, 此种的壳面较平, 而帕尔海链藻的壳面隆起呈鼓状; 第三, 此种的中央支持突具有3个围孔, 而帕尔海链藻具有4—5个围孔。Abstract: To explore the species diversity of the marine diatom genus Thalassiosira Cleve, several monoclonal strains were established from Daya Bay, north coast of the South China Sea. The morphology of the cells was examined by light and scanning electron microscopy. The hypervariable D1-D3 region of the nuclear large-subunit ribosomal DNA and the relatively conserved region of the nuclear small-subunit ribosomal DNA were sequenced and used for phylogenetic relationship analysis. Combined the morphology and molecular data, a new species, T. flabellata sp. nov. X H Guo, Y Q Guo & Y Li was described. The cells normally form short chains or solitary. A central fultoportula, one ring of marginal fultoportulae and one marginal rimoportula are present on the valve face. This species is characterized by fasciculate areolae on the valve.
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Keywords:
- Thalassiosira /
- Morphology /
- Phylogen /
- Thalassiosira flabellata
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图 1 基于核糖体小亚基SSU nrDNA构建的贝叶斯系统树
Figure 1. Molecular phylogenetic tree inferred based on sequences of SSU of the nuclear rDNA
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图 2 于核糖体大亚基LSU nrDNA D1-D3构建的贝叶斯系统树
Figure 2. Molecular phylogenetic tree inferred based on sequences of LSU of the nuclear rDNA
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图版Ⅰ 扇形海链藻
a—b. LM; c—j. SEM; a. 群体, 环面观; b. 壳面观; c. 外壳面观; d. 内壳面观; e. 中央支持突外壳面观; f. 中央支持突内壳面观; g. 外壳面唇形突和壳缘支持突; h. 内壳面唇形突和壳缘支持突; i. 壳缘支持突; j. 环面观及壳套
图版Ⅰ. Thalassiosira flabellata
a—b. LM; c—j. SEM. a. Colony in girdle view; b. Valve view; c. External valve view; d. Internal valve view; e. Central fultoportula on external valve; f. Central fultoportula on internal valve; g. Rimoportula and marginal fultoportulae on external vavle; h. Rimoportula and fultoportulae on internal valve; i. Marginal fultoportulae; j. Girdle view and valve mantle
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