功能Function | 基因Gene | 正向引物Forward primer (5′—3′) | 反向引物Reverse primer (5′—3′) |
紧密连接Tight junction | zo-1 | ACAAGAACAGGGCGGAACAGT | ACCTCCAGAAATCAGCACGA |
occludin-a | AGATGTGGAGGACTGGGTCA | ATTACGGACGGGCAGAATC | |
claudin-1 | CTGCTGTATCTGTGGGAGTGAA | TAATCAGGAGAACAGGCGAAG | |
claudin-b | GGTCCAGGCACAGAAGAGG | TTTTCGGGGCAGTGGCAACA | |
claudin-c | GGGGAAGTTTCTACCAGGAC | CTCTCACAAAGTCTGCCCAAG | |
claudin-12 | CATACAACACGGTGGAACCC | GTCAGCATCGCAATAACAAAGG | |
claudin-15a | CCATCTGTGGTGGAGTTTGTC | CATAAGTGCTCTGGGTTTCT | |
免疫相关Immune-related | nf-kb | GCGCTTTTCTGAATCCTACG | TGCCCAGTCTGTCTCCTTCT |
tlr4 | GTTCTTATGACGCATTTGTGAT | CGTTAGAGGCGATTGACTTCC | |
foxp3a | GCCTCCATGATACGATGGGCAAT | CCTTCCTTCAACACGCACAA | |
il-1β | TGGACTTCGCAGCACAAAATG | GTTCACTTCACGCTCTTGGATG | |
il-8 | TGTGTTATTGTTTTCCTGGCATTTC | GCGACAGCGTGGATCTACAG | |
tnf-alpha | GCGCTTTTCTGAATCCTACG | TGCCCAGTCTGTCTCCTTCT | |
tgf-beta | CAAACCAGCAGAGCACGGATA | CGGTGCCAACAGCTCGTCTC | |
il-10 | CACTGAACGAAAGTTTGCCTTAAC | TGGAAATGCATCTGGCTTTG | |
mmp9 | GCTCAACCACCGCAGACTAT | GTGCTTCATTGCTGTTCCCG | |
凋亡Apoptosis | caspase3 | CGGTGTAGGTGACGAGGAAA | TAAACCAGGAGCCATTAGCGA |
caspase8 | AAGACCTGATTCTGCGACTG | ATAGGCTGAGACACCTTTACG | |
caspase9 | CATCGCCCTCCTGTCATTTCT | CTGGCATCCATCTTGTAGCAC | |
细胞标记Cellmarker | gzma | GATTTTTGGCTGAGAGGACGG | ACGTCAGAGCAAACTGTCACT |
mpx | CTACATGGCACAAACGCTGAG | CTCGTCTTGAGTGAGCAGGTT | |
mpeg1 | TGCGGCACAATCGCAGTCCA | ACAGCAAAACACCCATCTGGCGA | |
lck | GCCGAAGAAGATCTCGATGGT | TCCCCATGTTTACGTATTTTGTCG | |
fabp2 | GAAGTCAGCACTTTCCGCAC | TGTGAAAGTCCCCTTGAGCG | |
内参House-keeping | rpl13a | GCCAACAGAGAGAAGATGACACAG | CAGGAAGGAAGGCTGGAAGAG |