Abstract: To investigate the role of carbon (δ¹³C) and oxygen (δ¹⁸O) stable isotopes in juvenile otoliths for identifying chum salmon (Oncorhynchus keta) groups, this study analyzed cultured and wild juvenile chum salmon groups (under 1 year old) using stable isotope mass spectrometry. The results showed that the mean δ¹⁸O and δ¹³C values in otoliths of the cultured group were (−10.33±0.30)‰ and (−8.60±0.45)‰, while those of the wild group were (−9.90±0.34)‰ and (−11.76±0.65)‰, respectively. Significant differences in otolith stable isotopes were observed between the two groups, primarily attributed to distinct freshwater habitat conditions experienced during their early life stages. By analyzing the correlation between δ¹⁸O and δ¹³C in juvenile chum salmon otoliths, cultured and wild juveniles were successfully discriminated, demonstrating the feasibility of juvenile otolith isotopes for tracing the origin of migratory adult chum salmon. This approach also provides a scientific basis for evaluating the effectiveness of artificial stock enhancement and its ecological impact on wild groups.