Abstract: In order to explore the effects of age and gender on the nutritional value of the calipash from the Chinese Soft-Shelled turtle (Pelodiscus sinensis), this study detected and analyzed the morphology index, basic nutritional components, amino acid content, and collagen fiber density of the calipash tissues from female and male Yellow River strain of Pelodiscus sinensis (the Yellow River strain of P. sinensis) age 2 to 6 years. The results showed that: (1) The ratios of calipash width/carapace width, calipash thickness/body height, and calipash weight/body weight differed significantly between males and females (P<0.05) of the Yellow River strain of P. sinensis. No significant difference was observed in calipash width/carapace width among different ages, whereas significant difference were found in calipash thickness/body height and calipash weight/body weight (P<0.05) of the Yellow River strain of P. sinensis. The calipash weight/body weight ratio showed significant differences across both age and gender (P<0.05) of the Yellow River strain of P. sinensis. (2) The moisture, ash, and crude protein content in the calipash of the Yellow River strain of P. sinensis differed significantly between males and females (P<0.05), while no significant difference was found in crude fat content. Among different ages, moisture, ash, and crude protein content of the calipash of the Yellow River strain of P. sinensis did not differ significantly, whereas crude fat content showed a significant difference (P<0.05). Significant differences were observed in moisture, ash, and crude protein content both age and gender of the calipash of the Yellow River strain of P. sinensis. (3) Total amino acid (TAA) content of the calipash of the Yellow River strain of P. sinensis differed significantly between males and females (P<0.05), but not among age groups. (4) The total essential amino acid content (ΣEAA), total semi-essential amino acid content (ΣSEAA), total non-essential amino acid content (ΣNEAA), and total delicious amino acid content (ΣDAA) of the calipash of the Yellow River strain of P. sinensis were significantly higher in females than in males (P<0.05). (5) The ΣDAA content of the calipash of the Yellow River strain of P. sinensis differed significantly among ages (P<0.05), while ΣEAA did not. Methionine + cysteine (Met + Cys) were identified as the limiting amino acids of the Yellow River strain of P. sinensis across all age groups. (6) Collagen fiber density in the calipash of the Yellow River strain of P. sinensis differed significantly between males and females(P<0.05), with males exhibiting a significantly higher collagen fiber density than females (P<0.05). Significant differences were also observed among ages, with 2 years old turtles exhibiting the lowest collagen fiber density (P<0.05) across all age groups. Age and gender had a significant interactive effect on collagen fiber density of the calipash of the Yellow River strain of P. sinensis (P<0.05). In conclusion, age and gender had a significant influence the nutritional value of the calipash of the Yellow River strain of P. sinensis. These findings provide a reference for optimizing aquaculture strategies and guiding consumer selection of the Yellow River strain of P. sinensis.