斜生栅藻对振荡和磷胁迫的生理生化响应
PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES OF SCENDESMUS OBLIQUUS TO PHOSPHORUS AND TURBULENCE STRESS
-
摘要: 为了探讨水文条件的变化对常见绿藻水华发生的潜在影响,实验以斜生栅藻为材料,研究了不同水流状态及磷浓度对其生长及磷利用策略的影响。实验分别设置静止条件(0 r/min)、低振荡条件(90 r/min)和高振荡条件(120 r/min),同时设置磷限制(0.2 mg/L)和磷充足(2 mg/L)两组磷浓度,整个实验过程为3周。实验过程测定的指标为:比生长速率、胞外碱性磷酸酶活性、叶绿素a含量以及磷吸收动力学参数。实验结果表明:(1)同静止条件相比,低振荡和高振荡条件均能显著降低斜生栅藻的比生长速率,但是两种不同磷浓度下其比生长速率却无显著性差异。(2)在实验第21天时,振荡条件培养下,斜生栅藻的胞外碱性磷酸酶活性显著高于静止条件培养,与此相反,胞内磷浓度却显著高于静止条件。(3)在磷限制条件下斜生栅藻的叶绿素a含量显著降低,同时两种振荡条件培养均使其在磷充足条件下叶绿素a含量降低。(4)通过磷吸收动力学参数的比较,静止条件培养的斜生栅藻对磷的亲和力高于其在振荡条件下。由此可见,斜生栅藻适应于静止无扰动且磷营养丰富的水体,随着水体中磷浓度进一步升高,静止和水流缓慢的水体存在导致像斜生栅藻这一类绿藻发生水华的风险。Abstract: In order to explore the potential effect of changing hydrological regimes on the green algae blooms, the effect of different levels turbulences on the growth and phosphorus (P) strategy of Scendesmus obliquus were studied. This experiment, lasting for 3 weeks, was designed with 3 levels turbulences, no turbulence(0 r/min), light turbulence (90 r/min)and high turbulence (120 r/min), combined with P-limit condition (0.2 mg/L) and P-replete condition (2 mg/L). The parameters determined during the experiment were listed as follows: growth rate, alkaline phosphatase activity (APA), cellular Chlorophyll a (Chl. a) contents and P uptake kinetic parameters. Our result showed: (1) Compared with the standing condition, light and high turbulence could both affect the growth rates of S. obliquus markedly, however, there was no significant variation between the growth rates of P-limit and P-replete condition. (2) On the 21st day, APAs of S. obliquus under turbulence were significantly higher than that under standing condition, contrarily, the cellular P concentrations of S. obliquus under turbulence were much higher than that under standing condition. (3) Chl. a contents of S. obliquus under P-limit condition declined significantly while light and high turbulences both leaded to the de-creasing of Chl. a contents under P-replete condition. (4) Through P uptake kinetic parameters, the affinity for P in S. obliquus under standing condition was higher than that under turbulence. According to our results, S. obliquus adapt to the standing and P-rich condition. Once the P concentration in waters continue to increase, the standing and low flowing rate water will face the risks that Green algae like S. obliquus will bloom.
-
Keywords:
- Scendesmus obliquus /
- Turbulence /
- Growth rate /
- P uptake /
- Alkaline phosphatase /
- Chlorophyll a
-
-
[1] Tang H B, Liu G X, Hu Z Y. Preliminary research on the algal bloom of Peridiniopsis sp. in Gaolan River of the Three Gorges Researvoir [J]. Acta Hydrobiologica Sinica, 2006, 30(1): 4752 [汤宏波, 刘国祥, 胡征宇. 三峡高岚河甲藻水华的研究. 水生生物学报, 2006, 30(1): 4752]
[2] Wang D, Huang C X, Huang B Q, et al. Physiological re-sponses of two typical species of diatoms to phosphorus stress in Yellow Sea [J]. Marine Sciences, 2008, 32(5): 2227 [王丹, 黄春秀, 黄邦钦, 等. 黄海两种典型硅藻的磷胁迫生理研究. 海洋科学, 2008, 32(5): 2227]
[3] Shen H, Song L R. Comparative studies on physiological responses to phosphorus in two phenotypes of bloom-form- ing Microcystis [J]. Hydrobiologia, 2007, 592(1): 475486
[4] Song G L, Li W, Cong C, et al. Study on the effect of Phos-phorus concentration in water body on the growth of Scenedesmus obliquus [J]. Journal of Anhui Agricultural Science, 2007, 35(35): 1157611577 [宋关玲, 李伟, 丛超, 等. 水体磷浓度对斜生栅藻生长影响的研究. 安徽农业科学, 2007, 35(35): 1157611577]
[5] Zeng H, Song L R, Yu Z G, et al. Post-impoundment bio-mass and composition of phytoplankton in the Yangtze River [J]. International Review of Hydrobiology, 2007, 92(3): 276280
[6] Xia J, Dong M, Zhang H. Dynamic model of eutrophication in Hanjiang River [J]. Chongqing Environmental Science, 2001, 23(1): 2023 [夏军, 窦明, 张华. 汉江富营养化动态模型研究. 重庆环境科学, 2001, 23(1): 2023]
[7] Stein J R. Handbook of Phycological Methods [M]. Cam-bridge: Cambridge University Press. 1979, 238
[8] Murphy J, Riley J P. A modified single solution method for determination of phosphate in natural waters [J]. Analytica Chimica Acta, 1962, 26(1): 3136
[9] Nishikawa T, Tarutani K, Yamamoto T. Nitrate and phos-phate uptake kinetics of the harmful diatom Eucampia zo-diacus Ehrenberg, a causative organism in the bleaching of aquacultured Porphyra thalli [J]. Harmful Algae, 2009, 8(3): 513517
[10] Eppley R W, Rogers J N, Mccarthy J J. Half-saturation con-stants for uptake of nitrate and ammonium by marine phyto-plankton [J]. Limnology and Oceanography, 1969, 14(6): 912920
[11] Healey F P. Slope of the monod equation as an indicator of advantage in nutrient competition [J]. Microbial Ecology, 1980, 5: 281286
[12] Peters F, Marrase C. Effects of turbulence on plankton: an overview of experimental evidence and some theoretical considerations [J]. Marine Ecology-Progress Series, 2000, 205: 291306
[13] Zhang M, Shi X L, Jiang L J, et al. Effects of the exogenous phosphorous and shake on the growth of Microcystis aeruginosa [J]. Chinese Journal of Applied and Environ-mental Biology, 2002, 8(5): 507510 [张民, 史小丽, 蒋丽娟, 等. 两种外源性磷及振荡对铜绿微囊藻(Microcystis aeruginosa)生长的影响. 应用与环境生物学报, 2002, 8(5): 507510]
[14] Zhu W, Wan L, Zhao L F. Effect of nutrient level on phyto-plankton community structure in different water bodies [J]. Journal of Environmental Sciences, 2010, 22: 3239
[15] Berdalet E. Effects of turbulence on the marine dinoflagellate Gymnodinium Nelsonii [J]. Journal of Phycology, 1992, 28: 267272
[16] Margulis L, Corliss, J O, Melkonian, M, et al. Handbook of Protoctista [M]. Boston: Jones and Bartlett Publishers, 1990, 134135
[17] Karpboss L, Boss E, Jumars P A. Nutrient fluxes to plank-tonic osmotrophs in the presence of fluid motion [J]. Oceanography and Marine Biology, 1996, 34(34): 71107
[18] Warnaars T A, Hondzo M. Small-scale fluid motion medi-ates growth and nutrient uptake of Selenastrum capricornu-tum [J]. Freshwater Biology, 2006, 51: 9991015
[19] Healey F P, Hendzel L L. Indicators of phosphorus and ni-trogen deficiency in 5 algae in culture [J]. Journal of the Fisheries Research Board of Canada, 1979, 36(11): 13641369
[20] Cotner J B, Wetzel R G. Uptake of dissolved inorganic and organic phosphorus-compounds by phytoplankton and bacte-rioplankton [J]. Limnology and Oceanography, 1992, 37(2): 232243
[21] Litchman E, Nguyen B L V. Alkaline phosphatase activity as a function of internal phosphorus concentration in freshwater phytoplankton [J]. Journal of Phycology, 2008, 44: 13791383
[22] Peters F, Arin L, Marrase C, et al. Effects of small-scale turbulence on the growth of two diatoms of different size in a phosphorus-limited medium [J]. Journal of Marine Systems, 2006, 61(3-4): 134148
[23] Giraudet H, Berthon J L, Buisson B. A comparison of the daily alkaline phosphatase activity of a cyanobacterium (Mi-crocystis aeruginosa) and a diatom (Synedra capitata) [J]. Comptes Rendus De L Academie Des Sciences Serie Iii-Sciences De La Vie Life Sciences, 1997, 320(6): 451458
[24] Hernandez I, Niell F X, Fernandez J A. Alkaline-phosphatase activity in Porphyra Umbilicalis (L) Kutzing [J]. Journal of Experimental Marine Biology and Ecology, 1992, 159(1): 113
[25] Hernndez I, Niell F X, Whitton B A. Phosphatase activity of benthic marine algae. An overview [J]. Journal of Applied Phycology, 2002, 14(6): 475487
[26] Falkner G, Falkner R, Graffius D, et al. Bioenergetic and ecological aspects of phosphate uptake by blue-green algae [J]. Archiv fr Hydrobiologie, 1984, 101: 8999
[27] Wykoff D D, Davies J P, Melis A, et al. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii [J]. Plant Physiology, 1998, 117: 129139
[28] Litchman E, Steiner D, Bossard P. Photosynthetic and growth responses of three freshwater algae to phosphorus limitation and daylength [J]. Freshwater Biology, 2003, 48(12): 21412148
-
期刊类型引用(6)
1. 魏晓雪，田晨，冯剑丰，朱琳. 磷限制条件下单胞藻胞内磷储量差异机理研究. 环境科学研究. 2022(06): 1398-1406 . 
百度学术
2. 陈敏怡，王振红，罗专溪. 不同磷源对砷胁迫下2种绿藻生长的影响. 生态毒理学报. 2020(05): 218-227 . 百度学术
3. 郐安琪，赵伟华，李青云，龙萌. 铜绿微囊藻对Cd~(2+)胁迫的生理生化响应. 人民长江. 2016(16): 20-25 . 百度学术
4. 杨宋琪，吴忠兴. 斜生栅藻在不同磷条件下氮生态幅的研究. 水生生物学报. 2014(03): 510-515 . 本站查看
5. 陈文捷，沈宏，庞兴红，吕丽媛，谢平. 三种水华藻类在振荡条件下的磷吸收动力学及生长预测研究. 长江流域资源与环境. 2013(S1): 59-65 . 百度学术
6. 吴叶宽，袁玲，黄建国，李隆云. 青蒿素对绿藻的化感效应. 中国中药杂志. 2013(09): 1349-1354 . 百度学术
其他类型引用(9)
计量
- 文章访问数:
- HTML全文浏览量: 0
- PDF下载量:
- 被引次数: 15
下载: