大黄鱼快长相关微卫星标记的筛选与验证

SCREENING AND VERIFICATION OF MICROSATELLITE MARKERS CLOSELY RELATED WITH RAPID GROWTH TRAITS IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA

  • 摘要: 为了筛选与大黄鱼生长性状紧密相关的分子标记,并对这些标记的有效性进行鉴定,研究以大黄鱼家系和群体为材料,经标记筛选、两次验证共三个步骤,找到2个与大黄鱼生长性状紧密相关的微卫星标记:LYC0088和LYC0143,其中LYC0088与体高、体质量均极显著相关(P0.01),与体长显著相关(P0.05),LYC0143与体长和体质量显著相关(P0.05),与体高的相关性极显著(P0.01)。LYC0088与LYC0143位点的优势等位基因分别为E、A,优势等位基因型均为AB,优势等位基因型组合为AB/AB,两个位点呈加性效应。该结果为开展大黄鱼分子标记辅助育种提供了有价值的遗传标记。

     

    Abstract: To screen the molecular markers closely related with growth traits and identify the validity of these markers in large yellow croaker, a family and a population were used as material and three steps were included. The first step was makers screening. In this step, a family with 60 extreme individuals was used as genetic material. The second step was markers preliminary verification. In this step, a family with 120 random individuals was used as genetic material. The last step was markers verification once again. A population with 60 extreme individuals was used. Two microsatellite markers closely related with growth traits were obtained. LYC0088 was significantly correlated with body height, body weight (P0.01), and body length (P0.05). LYC0143 was significantly correlated with body length and body weight (P0.05), and body height (P0.01). For LYC0088 and LYC0143 loci, the advantage allele was E and A, respectively. The advantage genotypes were both AB. The advantage combination of genotype was AB/AB. These two loci presented additive effects. The results provide valuable genetic markers for conducting large yellow croaker molecular marker-assisted breeding.

     

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