饲料蛋白水平对低温应激下吉富罗非鱼血清生化指标和HSP70 mRNA表达的影响
EFFECTS OF DIFFERENT DIETARY PROTEIN LEVELS ON SERUM BIOCHEMICAL INDICES AND EXPRESSION OF LIVER HSP70 mRNA IN GIFT TILAPIA (OREOCHROMIS NILOTICUS) UNDER LOW TEMPERATURE STRESS
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摘要: 在水温27℃条件下, 分别对吉富罗非鱼(27.642.79) g投喂3组不同蛋白水平的饲料, 养殖周期56d。饲养结束后, 进行14℃低温应激实验, 研究低温应激后0-24h内, 鱼体血清生化指标与肝脏HSP70 mRNA表达量的变化。结果表明, 短期投喂50%蛋白水平的饲料可以提高罗非鱼血清中葡萄糖、总蛋白、甘油三酯与胆固醇水平以及血清谷丙转氨酶与谷草转氨酶活力。在低温应激后, 50%蛋白组血清葡萄糖、总蛋白、甘油三酯与胆固醇水平呈下降趋势, 而血清皮质醇水平与谷草转氨酶活力表现为先上升后下降变化。25%和38%蛋白组血清葡萄糖水平与谷丙转氨酶活力在应激期间呈上升趋势, 而谷草转氨酶与碱性磷酸酶活力和总蛋白与胆固醇水平呈先上升后下降变化。各实验组溶菌酶活力与肝脏HSP70 mRNA的表达量呈先上升后下降的变化。由此可知, 短期投喂高蛋白饲料可以提高罗非鱼血液中蛋白、血糖与脂肪含量, 增强鱼体抗应激能力, 同时, 高蛋白水平的饲料也会引起肝脏产生分解压力以及增加饲料成本。在生产中, 需要根据实际需要合理确定饲料的蛋白含量。Abstract: Three Groups of GIFT tilapias (Oreochromis niloticus) were fed with protein levels at 25%, 38% and 50%, respectively at 27℃ in 56 days. The initial body weights were (27.642.79) g. Uniform sizes of fish were selected for low temperature stress test at the end of rearing management. Tilapias in the experiment group were subjected to 14℃, and some biochemical indices in their serum and the expression of HSP70 mRNA in liver were examined over 0-24h. The results showed that, before low temperature stress, the 50% protein level group had highest serum glucose (GLU), total protein (TP), triglycride and cholesterol level; Serum glutamic-pyruvic transaminase (ALT) and glutamic-oxaloacetic transaminase (AST) activities was also increased. After low temperature stress, in the 50% protein level group, serum glucose (GLU), total protein (TP), triglyceride and cholesterol level decreased within 24h, while glutamic-pyruvic transaminase (ALT) and glutamic-oxaloacetic transaminase (AST) activities increased at first and then gradually declined. In 25% and 38% protein groups, serum glucose (GLU) level and glutamic-pyruvic transaminase (ALT) activity increased within 24h, whereas serum ALT, alkaline phosphatase (AKP), TP and cholesterol level increased at first and then decreased. Serum lysozyme activity and expression level of HSP70 mRNA in liver of all groups also increased at first and then gradually declined. In conclusion, diet supplemented with high protein level could improve serum protein, blood sugar and lipid level and enhance resistance to low temperature, but could cause liver damage due to decomposition pressure; and could increase feed cost. Therefore, in production, the proportion of protein in the feed should be arranged according to the practical need of tilapia.
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Keywords:
- GIFT tilapia /
- Dietary protein /
- Low temperature stress /
- Serum /
- HSP70
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