重金属Cu2+、Pb2+单因子及联合毒性对泥鳅卵细胞DNA的损伤效应
EFFECTS OF Cu2+ AND Pb2+ (SINGLE FACTOR AND JOINT TOXICITY) ON DNA DAMAGE IN MISGURNUS ANGUILLICAUDATUS OOCYTES
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摘要: 采用室内暴露试验方法, 研究了不同浓度Cu2+(0.01、0.10、0.25mg/L)、Pb2+(0.05、0.50、0.75mg/L)单因子染毒以及Cu2++Pb2+(0.01 mg/L+0.05mg/L、0.10 mg/L+0.50 mg/L、0.25 mg/L+0.75 mg/L)联合染毒对泥鳅卵细胞DNA的损伤效应, 并以SCGE技术进行检测。结果显示, Cu2+与Pb2+单因子染毒对泥鳅卵细胞DNA的损伤具有较为显著的剂量-效应与时间-效应关系(P0.05)。Cu2++Pb2+联合染毒, 在溶液暴露的0-5d表现为剂量-效应与时间-效应关系(P0.05); Cu2++Pb2+暴露5-10d 则表现出拮抗作用。研究结果显示, Cu2+、Pb2+ 单因子及联合染毒均造成泥鳅卵细胞DNA损伤, 具有基因毒性效应。Abstract: The effects of Cu2+ and Pb2+ (single factor and joint toxicity) attack on DNA damages in loach oosytes with different concentration of Cu2+(0.01, 0.10 and 0.25 mg/L respectively)and Pb2+(0.05, 0.50 and 0.75mg/L respectively), and Cu2++Pb2+(0.01 mg/L+0.05mg/L, 0.10 mg/L+0.50 mg/L and 0.25 mg/L+0.75 mg/L respectively)were studied by means of in door exposure and with the technique of SCGE. The results showed that the DNA damages in loach oosytes by single factor Cu2+ or Pb2+showed significant differences with dose-effect and time-effect (P0.05). As for joint attack of Cu2++Pb2, it showed that there were significant differences with dose-effect and time-effect under 5d exposure (P0.05), but it seemed to be an antagonism within 5d to 10d exposure. The results of the study showed that both single factor or joint attack by heavy metals will cause DNA damages to loach oosytes. Meanwhile, the genetic toxicity effects appears.
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Keywords:
- Misgurnus anguillicaudatus /
- Oocyte /
- Cu2+ /
- Pb2+ /
- SCGE detection /
- DNA damage
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[1] Li L, Shen X Q, Wang Y L, et al. Kinetic study on the bioconcentration of Cu and Pb in two kinds of marine bivalve molluscs under the condition of sediment exposure[J]. Acta Hydrobiologica Sinica, 2012, 36(3): 522-531[李磊, 沈新强, 王云龙, 等. 在沉积物暴露条件下2 种海洋贝类对Cu、Pb 的富集动力学研究. 水生生物学报, 2012, 36(3): 522-531]
[2] Zhang Y M, Wang Y J, Yu R L, et al. Effects of heavy metals Cd2+, Pb2+ and Zn2+ on DNA damage of loach Misgurnus anguillicaudatus[J]. Acta Hydrobiologica Sinica, 2006, 30(4): 399-403[张迎梅, 王叶菁, 虞闰六, 等. 重金属Cd2+、Pb2+和Zn2+对泥鳅DNA损伤的研究. 水生生物学报, 2006, 30(4): 399-403]
[3] Has Schon E, Bougut I. Heavy metal concentration in fish tissues inhabiting waters of Busko Blato reservoir (Bosnia and Herzegovina)[J]. Environment Monitoring Assessment, 2007, 9(11): 1125-1130
[4] Gao X L, Qi F S, Luo H Y, et al. Acute and joint toxicity tests of Cu, Hg and Cr to loaches[J]. Reservoir Fisheries, 2003, 23(2): 63-64[高晓莉, 齐凤生, 罗胡英, 等. 铜、汞、铬对泥鳅的急性毒性和联合毒性实验. 水利渔业, 2003, 23(2): 63-64]
[5] Wang Y Q, Zhang Y M, Zhao D Q. Effects of heavy metals cadmium, lead and zinc on the survival of Carassius auratus and Misgurnus anguillicaudatu[J]. Journal of Gansu Sciences, 2003, 15(1): 35-38[王银秋, 张迎梅, 赵东芹. 重金属镉、铅、锌对鲫鱼和泥鳅的毒性. 甘肃科学学报, 2003, 15(1): 35-38]
[6] Fan L J, Zhou Z L, Chen D H, et al. Study on DNA damage induced by HgCl2 and CdCl2 using SCGE in Eriocheir sinensis sperms (in vitro)[J]. Journal of East China Normal University (Natural Science), 2007, 4: 95-100[范丽君, 周忠良, 陈东华, 等. HgCl2与CdCl2对河蟹精子DNA损伤的单细胞凝胶电泳检测研究. 华东师范大学学报(自然科学版), 2007, 4: 95-100]
[7] Xu X C, Ding F H, Li J. Cryopreservation caused sperm DNA damage in red sea bream Pagrosomus major and its detection[J]. Oceanologia et Limnologia Sinica, 2005, 36(3): 221-225[徐西长, 丁福红, 李军. 单细胞凝胶电泳用于检测低温保存的真鲷(Pagrosomus major)精子DNA损伤. 海洋与湖沼, 2005, 36(3): 221-225]
[8] Dang B J, Wang J, Du Q Y, et al. The DNA damage effect of paraquat on blood red cell of Paramisgurnus dabryanus[J]. Journal of Hydroecology, 2011, 32(5): 105-108[党炳俊, 王君, 杜启艳, 等. 单细胞凝胶电泳检测百草枯对大鳞副泥鳅血细胞DNA的损伤. 水生态杂志, 2011, 32(5): 105-108]
[9] Chen X, Hiroshi Nishida, Tetsuya Konishi. Oxidant-induced DNA damage and the reparation in NIH3T3 mouse fibroblasts by single cell gel electrophoresis[J]. Journal of Xi'an Jiaotong University (Medical Sciences), 2007, 28(1): 25-27[陈忻, 西田浩志, 小西徹也. 单细胞凝胶电泳技术检测小鼠成纤维细胞DNA的氧化损伤及修复. 西安交通大学学报(医学版), 2007, 28(1): 25-27]
[10] Jezierska B, Lugowska K, Witeska M. The effects of heavy metals on embryonic development of fish (a review)[J]. Fish Physiology and Biochemistry, 2009, 35(4): 625-640
[11] Abou El-Naga, E H, El-Mosehy K M, Hanmed M A.Toxicity of cadmium and copper and their effect on some biochemical parameters of marine fish mugil seheli. Egyptian[J]. Journal of Aquatic Resources, 2005, 31(2): 60-71
[12] Hproratsu MURANO, Kanae MATSUZAKI, Hiroaki SHIRAIFHI, et al. Effects of heavy metals in river waters in Japan on immobility and mortality of Daphnia magna and Oryzias latipes larvea[J]. Fisheries Science, 2007, 73: 1078-1086
[13] Tang J X, Xing C H, Liu Z L, et al. Accumulation of heavy metals (Cu and Pb) in the ovary of Misgurnus anguillicaudatus and the subsequent effects on ova development[J]. Oceanologia et Limnologia Sinica, 2010, 41(3): 386-390[唐建勋, 邢承华, 刘忠良, 等. 重金属Cu、Pb在泥鳅卵巢的蓄积特性及其对卵细胞发育的影响. 海洋与湖沼, 2010, 41(3): 386-390]
[14] Sun D W, Zhan Y, Xu Z R. The harmful effects of heavy metals to fishes[J]. Journal of Aquaculture, 2002, 15: 38-42[孙德文, 詹勇, 许梓荣. 重金属对鱼类的危害作用. 水产养殖, 2002, 15: 38-42]
[15] Zhou X W, Zhu G N, Sun J H, et al. Toxicity of copper, zinc, lead and cadmium to tissue's cellular DNA of the fish (Carassius auratus)[J]. Journal of Nuclear Agricultural Sciences, 2001, 15(3): 167-173[周新文, 朱国念, 孙锦荷, 等. Cu、Zn、Pb、Cd对鲫鱼(Carassius auratus)组织DNA毒性的研究. 核农学报, 2001, 15(3): 167-173]
[16] Ge Z Q, Qin W, Zhu Y F. The influence of embryonic development and fry survival to big whitebait by heavy metal ions Pb2+, Cu2+ and Cd2+[J]. Inland Fisheries, 2004, 11: 35-36[戈志强, 秦伟, 朱玉芳. 重金属离子Pb2+、Cu2+和Cd2+对大银鱼胚胎发育和仔鱼存活的影响. 内陆水产, 2004, 11: 35-36]
[17] Al-Weher S M. Levels of heavy metal Cd, Cu and Zn in three fish species collected from the North Jordan Valley, Jordan[J]. Jordan Journal of Biological Sciences, 2008, 1(1): 41-46
[18] Zhang C H, Lin Q D. The latest progress in single cell gel electrophoresis (SCGE) based on DNA damage detection[J]. Journal of Nuclear Agricultural Sciences, 2011, 2(6): 1230-1234[张春红, 林欣大. 单细胞凝胶电泳检测DNA损伤的最新进展. 核农学报, 2011, 2(6): 1230-1234]
[19] Ge Y M, Ning H M, Li J X. Application of single cell gel electrophoresis in clinical veterinary medicine[J]. Progress in Veterinary Medicine, 2007, 28(12): 77-80[葛亚明, 宁红梅, 李敬玺, 等. 单细胞凝胶电泳技术在临床兽医学中的应用. 动物医学进展, 2007, 28(12): 77-80]
[20] Hu X P, Zhou J H, Shi X J. Detection of DNA damage in Carassius auratus lymphocytes induced by cadmium with single cell gel electrophoresis assay[J]. Journal of Agro-environmental Science, 2005, 24(1): 43-45[胡晓磐, 周建华, 时夕金. 利用单细胞凝胶电泳技术研究镉对鲫鱼淋巴细胞DNA的损伤. 农业环境科学学报, 2005, 24(1): 43-45]
[21] Lu H X, Xu Y J. Effects of cadmium on antioxidant enzyme activity and DNA damage in Sinonovacula constricta[J]. Marine Environmental Science, 2011, 30(1): 96-101[陆慧贤, 徐永健. 镉胁迫下缢蛏(Sinonovacula constricta)抗氧化酶活性及DNA损伤的研究. 海洋环境科学, 2011, 30(1): 96-101]
[22] Ma G Y, Sun K J, Wang J P, et al. Effects of the interaction of heavy metals on the accumulation of lead and copper in Carassius auratus[J]. Chinese Journal of Spectroscopy Laboratory, 2005, 22(5): 983-986[马桂云, 孙开进, 王京平, 等. 混合重金属离子相互作用对Cu和Pb在鲫鱼体内积累的影响. 光谱实验室, 2005, 22 (5): 983-986]
[23] Ding W Q, Liu D Q, Ge F, et al. The molecular mechanism by heavy metal stress in fish[J]. Journal of Biology, 2012, 29(2):84-87[丁为群, 刘迪秋, 葛锋, 等. 鱼类对重金属胁迫的分子反应机理. 生物学杂志, 2012, 29(2): 84-87]
[24] Lei Y Z. Water Environmental Chemistry of Aquaculture[M]. Beijing: China Agriculture Press. 2004, 251-260[雷衍之. 养殖水环境化学. 北京: 中国农业出版社. 2004, 251-260]
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