翘嘴红鲌(♀)×黑尾近红鲌(♂)杂种F1的AFLP分析

ANALYSIS OF THE HYBRID F1 BETWEEN ERYTHROCULTER ILISHAEFORMIS♀×ANCHERYTHERYTHROCULTER NIGROCAUDA♂ BYAFLP MARKER TECHNIQUE

  • 摘要: 采用AFLP分子标记技术对翘嘴红鲌(♀)、黑尾近红鲌(♂)及杂种F1三个群体各12个个体进行遗传差异分析。结果表明: 9对引物共扩增出257条带, 多态性比例为51.36%; 三个群体间特异条带共24条, 其中黑尾近红鲌群体和杂种F1群体共有特异带15条、翘嘴红鲌群体和杂种F1群体共有特异带2条、翘嘴红鲌群体特异带7条; 黑尾近红鲌群体、翘嘴红鲌群体及杂种F1群体的Shannon's信息指数分别为0.2326、0.0794和0.1774, Nei's基因多样性指数分别为0.1602、0.0537和0.1229, 期望杂合度分别为0.1582、0.0464和0.1062; 杂种F1群体和黑尾近红鲌群体及翘嘴红鲌群体遗传距离分别为0.0523、0.2165, 遗传相似系数分别为0.9490、0.8053, 遗传数据表明杂种F1更接近于父本黑尾近红鲌。

     

    Abstract: AFLP molecular marker technique was utilized to analyze the genetic differences among Erythroculter ilishaeformis, Ancherythroculter nigrocauda and their hybrid F1 (E. ilishaeformis♀×A. nigrocauda; 12 individuals each). A total of 257 bands were obtained by 9 primer combinations, and the percentage of polymorphic loci was 51.36%. There were 24 specific bands in the three populations. There were 15 shared specific bands between the A. nigrocauda and hybrid F1, and 2 were between the E. ilishaeformis and hybrid F1. There were 7 specific bands of E. ilishaeformis. The Shannon's information indexes of A. nigrocauda, E. ilishaeformis and hybrid F1 were 0.2326, 0.0794 and 0.1774, respectively. The Nei's gene diversity indexes of the three populations were 0.1602, 0.0537 and 0.1229, respectively. The expected heterozygosity among the three populations was 0.1582, 0.0464 and 0.1062, respectively. The genetic distance was 0.0523 between the A. nigrocauda and hybrid F1 and was 0.2165 between the E. ilishaeformis and hybrid F1. The genetic similarity coefficients between the A. nigrocauda and hybrid F1 was 0.9490, and it was 0.8053 between the E. ilishaeformis and hybrid F1. These data showed that the hybrid F1 was more homogeneous to the male parent of A. nigrocauda.

     

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