微颗粒饲料中添加精胺对半滑舌鳎稚鱼生长和肠道发育的影响

THE EFFECT OF MICRODIET SUPPLEMENTATION WITH SPERMINE ON THE GROWTH AND INTESTINAL DEVELOPMENT OF TONGUE SOLE POSTLARVAE

  • 摘要: 为获知微颗粒饲料中添加精胺对半滑舌鳎仔稚鱼肠道发育的影响,试验以添加0,0.10%,0.33%精胺的微颗粒饲料(F1、F2、F3)和活饵料卤虫(F4)饲喂初始体长为2 cm左右的半滑舌鳎稚鱼。养殖28d后结果表明,卤虫组(F4)的特定生长率最高,饲料组中F2组特定生长率要显著高于F1和F3组(P0.05)。F3组的存活率仅为60.27%,显著低于其他各组(P0.05)。消化酶活力测定结果中,F2组在14d和28d时都含有较高的碱性磷酸酶比活力和亮氨酸氨基肽酶比活力,较低的亮氨酸-丙氨酸肽酶比活力。卤虫组的肠道发育情况最好,微绒毛长度显著大于其他各组(P0.05);黏膜厚度略小于F2组,但是没有显著性差异(P0.05);饲料组中F2组微绒毛长度和黏膜厚度都显著大于F1和F3组(P0.05)。研究表明,在微颗粒饲料中添加0.10%的精胺(F2组)对半滑舌鳎稚鱼肠道发育有促进作用。

     

    Abstract: To study the effects of spermine on the intestinal development of Cynoglossus semilaevis postlarvae, we selected the subject with initial length of about 2 cm and fed them with microdiets containing 0 (F1), 0.10% (F2), 0.33% (F3) of spermine and live Artemia (F4) for 28 days. The results showed that F4 group exhibited the highest growth and survival rates. The specific growth rate of F2 group was significantly higher than that of F1 and F3 groups (P0.05). The survival rate of F3 group was 60.27%, which was significantly lower than that of other groups (P0.05). At day 14, the alkaline phasphatase relative activities (AP) in the bodies of fish fed with spermine-containing microdiet were significantly higher than that in fish fed with the Artemia (P0.05); there was no significant difference among groups fed with spermine-containg mecrodiets (P0.05). At day 28, the AP of fish fed with 0.10% spermine was significantly higher than other groups (P0.05); no significant difference was found among other groups (P0.05). F2 and F3 groups showed higher leucine aminopeptidase (LAP) specific activities than F1 and F4 groups at day 14 and 28 (P0.05), but there was no significant difference between F2 and F3 (P0.05). F2 group showed the lowest leucine-alanine peptidase (leuala) specific activity at day 14 and 28 (P0.05). The microvilli length of Artemia group was significantly greater than that of the other groups (P0.05). The mucosal layer of Artemia group was apparently but insignificantly hinner than that of F2 group (P0.05). Both the length of microvilli and the thickness of the mucosal layer in F2 were greater than those of F1 and F3 groups (P0.05). These results suggested that 0.10% spermine in microdiet had positive effects on the intestinal development of tongue sole postlarvae.

     

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