玉屏风散对鱼类非特异性免疫和免疫相关基因表达的影响
THE EFFECTS OF JADE SCREEN POWER ON THE NON-SPECIFIC IMMUNE RESPONSE AND THE EXPRESSION OF THE RELATED GENES IN FISH
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摘要: 为探究传统中药玉屏风散在水产养殖上的生物学功效,在基础饲料中分别添加2.5%、5%和10%的玉屏风散和1%的芪参散配成实验饲料,饲喂实验鱼2周,然后分别观察两种中药对罗非鱼(体重约220 g)的生长、非特异性免疫及免疫相关基因表达的影响以及对草鱼(体重约20 g)免疫保护率的影响。结果显示:各中药组均提高了罗非鱼增重率, 10%玉屏风散组效果最显著;各实验组中罗非鱼肝、肾、脾体指数和各血液学指标与对照组无显著差异;各中药组罗非鱼谷草转氨酶(AST)和谷丙转氨酶(ALT)含量均不高于空白对照组; 2.5%、10%玉屏风散组和1%芪参散组碱性磷酸酶(AKP)含量均高于对照组(P0.05);各药物组罗非鱼溶菌酶活性均高于对照组,且2.5%玉屏风散组与对照组差异显著(P0.05);玉屏风散各剂量组罗非鱼呼吸暴发活性均高于对照组(P0.05);罗非鱼肝脏中热休克蛋白70(HSP70)在各实验组中表达量均上调,肾脏中HSP70在2.5%和5%玉屏风散组表达量上调,脾脏中HSP70在各实验组中表达量均下调;肝脏中转化生长因子受体TGFRⅢ在2.5%玉屏风散组表达量上调,肾脏中TGFRⅢ在2.5%玉屏风散组和1%芪参散组表达量上调,脾脏中TGFRⅢ在各实验组中表达量均上调;玉屏风散各剂量组能够提高草鱼对嗜水气单胞菌的抵抗能力,表现为死亡率降低和进入血液的细菌减少,其中5%玉屏风散组效果最佳。实验表明,玉屏风散能够有效促进罗非鱼生长,对其非特异性免疫指标以及免疫相关基因表达均有不同程度的促进作用,并能够有效地提高草鱼的免疫保护率。Abstract: In this study, we investigated the effects of Jade screen powder on the non-specific immune response and the expression of the related genes in tilapia(Oreochromis niloticus) as well as the effects on the resistance to the infection in grass carps. In order to explore the bio-activity of traditional Chinese herb Jade screen powder on aquaculture, the fish was fed with the four groups of diets supplemented with Chinese herbs(2.5%, 5%, 10% Jade screen powder and 1% Qishen powder) for 2 weeks. For tilapia, all four Chinese herb groups exhibited increase in the rate of the body weight gain, and 10% Jade screen powder had the most significant effect. There was no difference in the index of liver, kidney, spleen and in the haematological parameters between the treated groups and the control. Compared to the control group, the levels of AST and ALT were lower in the treated groups, but the level of AKP in the 2.5% and 10% Jade screen powder groups and 1% Qishen powder group was higher. There was an insignificant increase in the activity of the serum lysozyme in tilapia treated with jade screen powder at all three concentrations. Moreover, the activity of plasma lysozyme increased significantly in the group fed with 2.5% Jade screen powder compared to the control(P0.05). The herbal treatment also insignificantly elevated the production of ROS(reactive oxygen species)(P0.05). The expression of HSP70 was affected by the herbal treatments in a tissue-specific pattern:it was up-regulated in the liver, and in the kidney 2.5% and 5% Jade screen powder boosted the expression, however the expression was down-regulated in the spleen. The expression of TGFRⅢ was also differently regulated by the herbs in various organs:2.5% Jade screen powder up-regulated the expression in the liver and the kidney, and 1% Qishen powder also had a stimulating effect in the kidney, and the expression could be enhanced by all treatments in spleen. Jade screen powder improved the resistance to the infection of Aeromonas hydrophila in grass carps, which was represented by the lower mortality rate and less bacteria in the blood, and 5% Jade screen powder was especially effective. These results suggested that Jade screen powder may improve the growth, the non-specific immune response and the expression of related genes in tilapia.
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[1] Wang D, Li S W, Lu T Y, et al. Effect of Jade screen power on the blood biochemical indicators and non-specific immunity of Acipenser schrencki[J]. Journal of Northwest AF University, 2012, 40(3):39-46[王狄,李绍戊,卢彤岩,等.玉屏风散对施氏鲟血液生化指标及非特异性免疫功能的影响.西北农林科技大学学报, 2012, 40(3):39-46]
[2] Zhao H Y, Xu Q H, Wang Y F, et al. Effect of the biotransformatic mixture of jade screen power on the function of humoral immunity in immunosuppreessed mice[J]. Acta Chinese Medicine and Pharmacology, 2010, 38(5):26-29[赵红晔,徐启华,王月飞,等.玉屏风散生物转化液对免疫抑制小鼠体液免疫功能的影响.中医药学报, 2010, 38(5):26-29]
[3] Liang C M, Wang X X, Dong Q, et al. A serum pharmacologiacal study on the immunoregulatory effects of Yupingfeng Pulvis in mice[J]. Shanghai Journal of Immunology, 2003, 23(6):385-388[梁春敏,王贤喜,董群,等.玉屏风散对小鼠免疫调节作用的血清药理学研究.上海免疫学杂志, 2003, 23(6):385-388]
[4] Yang H, Chen Z L, Deng Y. Effect of Plus Yupingfeng Powder on immune function of red blood cell and oxygen free radicals in chickens[J]. Veterinary Science in China, 2006, 36(1):70-73[杨鸿,陈杖榴,邓桦.加味玉屏风散对鸡红细胞免疫和自由基的影响.中国兽医科学, 2006, 36(1):70-73]
[5] Yan X L. Allergic rhinieis treated with Modified Yu Ping Feng San in 115 cases[J]. Journal of Beijing University of Traditional Chinese Medicine, 2011, 34(5):358-360[闫锡联.玉屏风散加味治疗变应性鼻炎115例临床观察.北京中医药大学学报, 2011, 34(5):358-360]
[6] Liu T Z, Zhang X K, Wang G C, et al. Effects of different chinese herbal compounds on growth, nonspecific immunity and lymphocyte transformation in common carp Cyprinus carpio[J]. China Academic Journal Electronic, 2011, 30(8):445-450[刘铁铮,张小葵,王桂春,等.不同中药组方对鲤鱼生长、非特异性免疫及淋巴细胞转化的影响.水产科学, 2011, 30(8):445-450]
[7] Christybapita D, Divyagnaneswari R, Dinakaran M R. Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus[J]. Fish Shellfish Immunology, 2007, 23:840-852
[8] Vera-Jimenez N I, Pietretti D, Wiegertjes G F, et al. Comparative study of -glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminal-enhanced chemiluminescence assay in common carp(Cyprinus carpio L.)[J]. Fish Shellfish Immunology, 2013, 34:1216-1222
[9] Truter J C, va Wyk J H, oberholster P J, et al. The impacts of neutralized acid mine drainage contaminated water on the expression of selected endocrine-linked genes in juvenile Mozambique tilapia Oreochromis mossambicus exposed in vivo[J]. Ecotoxicology and Environmental Safety, 2014, 100:209-217
[10] Liu A J, Leng X J, Li X Q, et al. Effect of mannan oligosaccharides on growth performance, intestinal structure and nonspecific immunity of tilapia, Oreochromis niloticus[J]. Journal of Zhejiang University(Agric Life Sci), 2009, 35(5):329-336[刘爱君,冷向军,李小勤,等.甘露寡糖对奥尼罗非鱼生长、肠道结构和非特异性免疫的影响.浙江大学学报(农业与生命科学版), 2009, 35(5):329-336]
[11] Liu X G, Song L P, Zhou H Q. The effect of SMW-chitosan on the oxidation resistance capability and fat content in tne liver tissue of Tilapia nilotica[J]. Marine Fisheries, 2004, 26(4):291-294[刘兴国,宋理平,周洪琪.低分子壳聚糖对罗非鱼肝组织抗氧化能力和肝脂含量影响的研究.海洋渔业, 2004, 26(4):291-294]
[12] Yildirima M, Lima C, Wanb Peter J, et al. Growth performance and immune response of channel catfish(Ictaluruspuctatus) fed diets containing graded levels of gossypolacetic acid[J]. Aquaculture, 2003, 219(1-2):751-768
[13] Baldi A, Bontempo V, Dell'orto V, et al. Effects of dietary chromium-yeast in weaning-stressed piglets[J]. Canadian Journal of Animal Science, 1999, 79(3):369-374
[14] Meng Z N, Chen Y C, Guan X T, et al. Effect of Chinese herb compounds on activities of transaminase in-serum and antioxidase in erythrocyte of Cyprinus carpio L[J]. Journal of Northeast Agricultural University, 2010, 41(8):75-80[孟兆娜,陈玉春,管雪婷,等.复方中草药对镜鲤(Cyprinus carpio L.)血清转氨酶及红细胞抗氧化酶活性的影响.东北农业大学学报, 2010, 41(8):75-80]
[15] Sun H M. Effect of starvation on the blood indices and immunity function of Yellow catfish(Pseudobagrus fulvidraco Richardson)[D]. Thesis for Master of Science. College of animal science and technology, Jilin Agricultural University, Jilin. 2004[孙红梅.饥饿对黄颡鱼血液指标及免疫机能的影响.硕士学位论文,吉林农业大学动物科技学院,吉林. 2004]
[16] Li Q, Xiao D Y, Mao H M. The effect of bio-active peptide on the blood physiological-biochemical index in carp[J] Journal of Yangtze University(Nat Sci Edit), 2005, 2(5):27-29[李清,肖调义,毛华明.生物活性肽对鲤鱼血液生理生化指标的影响.长江大学学报(自然科学版), 2005, 2(5):27-29]
[17] Nie G X, Ming H, Zheng J L, et al. Effects of xylanase on blood physiological-biochemical parameters of Nile tilapia Oreochromis niloticus[J]. Journal of DaLian Fisheries Univeristy, 2007, 22(5):361-365[聂国兴,明红,郑俊林,等.木聚糖酶对尼罗罗非鱼血液生理生化指标的影响.大连水产学院学报, 2007, 22(5):361-365]
[18] Alexander J B, Ingram G A. Non-cellular and non-specific defense mechanisms of fish[J]. Annual Review of Fish Diseases, 1992, 2:249-280
[19] Wu Y R, Gong Q F, Fang H, et al. Effect of Sophora flavescens on non-specific immune response of tilapia(GIFT Oreochromis niloticus) and disease resistance against Streptococcus agalactiae[J]. Fish Shellfish Immunology, 2013, 34:220-227
[20] Lszl Ard, Yin G J, Xu P, et al. Chinese herbs(Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia(Oreochromis niloticus) and resistance against Aeromonas hydrophila[J]. Aquaculture, 2008, 275:26-33
[21] Liu M, Pan J L, Zhang S R. Phagocytes and phagocytosis in fish[J]. Ludong University Journal, 2010, 26(2):167-172[刘敏,潘俊丽,张士璀.鱼类吞噬细胞和吞噬作用的研究进展.鲁东大学学报, 2010, 26(2):167-172]
[22] Vera-Jimenez N I, Pietretti D, Wiegertjes G F, et al. Comparative study of -glucan induced respiration burst measured by nitroblue tetrazolium assay and real-time luminal-enhanced chemiluminescence assay in common carp(Cyprinus carpio L.)[J]. Fish Shellfish Immunology, 2013, 34:1216-1222
[23] Yin G J, Ardo L, Thompson K D, et al. Chinese herbs(Astragalus radix and Ganoderma lucidum) enhanced immune response of carp, Cyprinuscarpio, and protection against Aeromonas hydrophila[J]. Fish Shellfish Immunology, 2008, 275:26-33
[24] Iwama G K, Afonso L O, Todgham A, et al. Are hsps suitable for indicating stressed states in fish[J]. Experimental Biology, 2004, 207:15-19
[25] Ming J H, Xie J, Xu P, et al. Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream(Megalo-brama amblycephala Yih) under high temperature stress[J]. Fish Shellfish Immunol, 2012, 32:651-661
[26] Lei A Y, Zeng D G. Effects of compound Chinese herbal on the expression of heat stress protein 70 gene in white shrimp(litopenaeus vannamei)[J]. Guangxi Agricultural Sciences, 2008, 6:830-833[雷爱莹,曾地刚.复方中草药对凡纳滨对虾热应激蛋白70基因表达的影响.广西水产科技, 2008, 6:830-833]
[27] Xiang S W, Huang G D, Huang R F, et al. Effect of compound xiancao granules on expression of TGF-1 and TR-Ⅱ in renal tissue among rats with IgA nephropathy[J]. Journal of Anhui TCM College, 2013, 32(4):65-69[向少伟,黄国东,黄仁发,等.复方仙草颗粒对IgA肾病大鼠转化生长因子及其Ⅱ型受体表达的影响.安徽中医学院学报, 2013, 32(4):65-69]
[28] Sun Y, Song J X, Wang Z M, et al. Expression of TGF-betaRⅠ, Smad2, Smad3 and Smad7 in keloids[J]. Chinese Journal of Plastic Surgery, 2006, 22(5):368-370
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