水华鱼腥藻类菌胞素氨基酸的分子鉴定和化学检测

IDENTIFICATION AND DETERMINATION OF MYCOSPORINE-LIKE AMINO ACIDS (MAAS) IN DOLICHOSPERMUM FLOS-AQUAE

  • 摘要: 类菌胞素氨基酸 (MAAs) 是一类具有吸收紫外线能力的物质, 蓝藻MAAs的生物合成及其分子机制的揭示为MAAs基因的快速检测提供了可能。研究采用分子生物学方法扩增了一株分离自太湖的水华鱼腥藻(Dolichospermum flos-aquae CHAB1629)编码脱氢醌合成酶(DHQS)基因的部分片段, 系统树分析发现其与报道的Anabaena sp. 90核酸序列相似度达99%, 而与Anabaena variabilis ATCC 29413仅为53.6%; 同时运用HPLC检测发现, 该株水华鱼腥藻MAAs的类型为shinorine。研究结果可为后续浮游类鱼腥藻MAAs的分子鉴定及野外适应性研究提供依据。

     

    Abstract: Mycosporine-like aminoacids (MAAs) exist in cyanobacteria, fungi, and eukaryotic micro-and macro-algae. They absorb ultraviolet (UV) and hence protect these organisms from the damages caused by the environmental UV radiation. The understanding of the biosynthetic pathway and the genetic properties of MAAs is fundamental for the fast-detection of MAAs genes. In the present study, we amplified the partial coding sequence of the gene encoding DHQS enzyme in Dolichospermum flos-aquae CHAB1629 that was isolated from the Taihu Lake. The nucleotide similarity between D. flos-aquae CHAB1629 and Anabaena sp. 90 was 99%, and it was only 53.6% between D. flos-aquae CHAB1629 and Anabaena variabilis ATCC 29413. High-performance liquid chromatography (HPLC) assays confirmed that MAAs in D. flos-aquae CHAB1629 were shinorine.

     

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