饲料中添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽和抗氧化能力的影响

EFFECTS OF DIETARY SUPPLEMENTATION OF SYNTHETIC ASTAXANTHIN ON OVARIAN DEVELOPMENT, COLORATION AND ANTIOXIDANT CAPACITY OF ADULT FEMALE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS

  • 摘要: 采用在基础饲料中分别添加0、130和260 mg/kg的合成虾青素配制成3种粗蛋白和粗脂肪含量分别为42%和16%的等氮等脂的中华绒螯蟹(Eriocheir sinensis)育肥饲料(分别记为饲料1、2和3), 以中华绒螯蟹商业育肥饲料作为饲料4, 分别投喂4组雌蟹(每个饲料组3个重复水槽, 每个水槽中12只蟹), 进行为期60d的室内养殖实验, 以探讨添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽及抗氧化能力的影响。结果显示: (1)实验进行到30d和60d, 在饲料中添加虾青素对雌体肝胰腺指数(HSI)和性腺指数(GSI)均无显著影响(P>0.05)。(2)性腺、肝胰腺和头胸甲中的总类胡萝卜素含量和红度值(a值)均以饲料3组最高, 性腺亮度值(L值)和黄度值(b值)以饲料1组最高(P<0.05); 饲料2组头胸甲的b值最高, 饲料1组最低(P<0.05)。(3) 饲料1组中华绒螯蟹血清过氧化物酶(POD)活性显著高于其他组, 饲料4组的过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)、乳酸脱氢酶(LDH)、总抗氧化能力(T-AOC)、丙二醛(MDA)和乳酸(LD)含量最高(P<0.05); 肝胰腺中的SOD、T-AOC、GSH-Px和GR活性均以饲料1组最高, 饲料2组最低(P<0.05)。(4) 饲料2组血清酸性磷酸酶(ACP)活性和血蓝蛋白(Hc)含量显著高于其他组, 其余各组间差异不显著。血清中的碱性磷酸酶(ALP)和γ-谷氨酰转移酶(γ-GT)活性和一氧化氮(NO)含量均以饲料4组最高(P<0.05), 肝胰腺中的ACP、ALP和γ-GT活性以饲料1组最高。综上, 在育肥饲料中添加合成虾青素对成体雌蟹性腺发育无显著影响, 但可显著提高头胸甲、肝胰腺和卵巢中的类胡萝卜素总量、色泽和抗氧化能力, 建议雌蟹育肥饲料中合成虾青素的含量为90 mg/kg左右。

     

    Abstract: To investigate the effects of dietary supplementation of synthetic astaxanthin on ovarian development, coloration and antioxidant capacity of adult female Chinese mitten crab (Eriocheir sinensis), three isonitrogenous and isolipidic fattening diets with 42% crude protein and 16% crude lipid were formulated by adding 0, 130 and 260 mg/kg synthetic astaxanthin (named as Diet 1, Diet 2, and Diet 3, respectively), and commercial fattening diet was used (named as Diet 4). Each diet was fed to triplicate tanks with 12 female crabs per tank for 60-day fattening. The results showed that dietary supplementation of synthetic astaxanthin had no significant effect on the hepatosomatic index (HSI) and gonadosomatic index (GSI) of adult female E. sinensis (P>0.05). Diet 3 treatment had the highest contents of carotenoid and redness value (a), and Diet 1 group had the highest lightness value (L) and yellow value (b) in the ovaries. The highest and the lowest b value of carapace were found in Diet 2 and Diet 1 treatments, respectively (P<0.05). Diet 1 group had the highest activity of peroxidase (POD) in the serum. The activities of catalase (CAT), glutathione reductase (GR), lactic dehydrogenase (LDH), total antioxidant capacity (T-AOC) and the contents of malondialdehyde (MDA) and lactic acid (LD) in serum from Diet 4 group were significantly higher than those of other groups (P<0.05). The highest and lowest activities of superoxide dismutase (SOD), T-AOC, glutathione peroxidase (GSH-Px) and GR in hepatopancreas were found in Diet 1 treatment and Diet 2 treatment, respectively (P<0.05). The activity of acid phosphatase (ACP) and hemocyanin (Hc) content in the serum from Diet 2 treatment were signifi-cantly higher than those of other treatments (P<0.05). The highest activities of alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT), and nitric oxide (NO) content were found in Diet 4 treatment. The highest activities of ACP, ALP and γ-GT in the hepatopancreas were shown in Diet 1 group. In conclusion, dietary supplementation of synthetic astaxanthin did not significantly affect the ovarian development of adult female crabs, but could enhance content of carotenoid, coloration and antioxidant capacity. These results suggest that the appropriate content of synthetic astaxanthin in the fattening diet is 90 mg/kg for female E. sinensis.

     

/

返回文章
返回