棘胸蛙致病性蜡样芽孢杆菌的分离鉴定及病理组织观察

ISOLATION AND IDENTIFICATION OF BACILLUS CEREUS AND PATHOLOGICAL OBSERVATION FROM ROTTEN-SKIN DISEASE OF QUASIPAA SPINOSA

  • 摘要: 试验从患烂皮病棘胸蛙(Quasipaa spinosa)中筛选出3种致病性菌株BS1、BS2和BS3, 对其进行革兰氏染色、形态观察、生理生化鉴定和16S rDNA序列分析, 鉴定上述3株菌均为蜡样芽孢杆菌。同时观察人工感染棘胸蛙的组织病理切片, 发现经病原菌感染的棘胸蛙肝、肺、舌和肌肉的损伤较严重, 表现出明显变性、坏死和炎症细胞浸润, 且在肺部组织内发现嗜碱性包涵体。药敏试验表明, 在所试的7种抗生素中, 致病性蜡样芽孢杆菌对庆大霉素和链霉素高度敏感, 对妥布霉素、卡那霉素和四环素中度敏感, 对氨苄西林和头孢他啶产生抗药性。实验初步探究了棘胸蛙烂皮病的发病机理, 为棘胸蛙烂皮病的防治提供理论依据。

     

    Abstract: Rotten-skin disease is very common in the artificial culture of the giant spiny frog (Quasipaa spinosa). To screen pathogenic bacteria that cause illness of giant spiny frog, gram stain, morphological observation, physiological, and biochemical identification, and sequence analysis of 16S rDNA were performed and three bacteria (BS1, BS2, and BS3) were identified. The results showed that BS1, BS2, and BS3 are Bacillus cereus. Liver, lung, tongue, and muscle were impaired with degeneration, necrosis, and inflammatory cell infiltration. Moreover, basophilic inclusions were observed in the lung of diseased giant spiny frog. The drug sensitivity test showed that all three pathogenic bacteria were highly sensitive to gentamicin and streptomycin, moderate sensitive to tobramycin, kanamycin and tetracycline, and resistant to ampicillin and ceftazidime. This study explored pathogenesis of rotten-skin disease of giant spiny frog, would provide theoretical basis for prevention and cure of rotten-skin disease of giant spiny frog.

     

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