环境激素睾丸酮和雌二醇对卜氏晶囊轮虫繁殖影响

EFFECTS OF ENVIRONMENTAL HORMONES TESTOSTERONE AND ESTRADIOL ON REPRODUCTION OF FRESHWATER ROTIFER ASPLANCHNA BRIGHTWELLI

  • 摘要: 采用生态学实验方法, 研究睾丸酮(Testoterone)和雌二醇(Estradiol)单一及组合对卜氏晶囊轮虫生命周期中主要发育阶段历时和种群增长率的影响。结果显示: 睾丸酮(T)的48h LC50为9.56 mg/L, 直线回归方程为Y=1.98X+3.06 (R2=0.92); 雌二醇(E) 48h LC50为8.18 mg/L, 直线回归方程为Y=3.53X+1.78 (R2=0.92)。除0.5 mg/L浓度组外, 睾丸酮和雌二醇均显著缩短轮虫平均寿命、减少后代幼体个数以及降低种群增长率(r)。其中8 mg/L睾丸酮与对照组相比显著延长轮虫生殖前期11.71%、平均寿命缩短52.22%, 后代个数减少82.20%; 8 mg/L雌二醇与对照组相比, 生殖前期显著延长7.10%, 平均寿命缩短49.75%, 后代个数减少83.33%。在2种激素定量组合(8 mg/L)实验中, 与对照组相比, 各实验组的生殖前期显著延长34.17%—48.01%, 平均寿命显著缩短31.56%—42.12%, 后代个数明显减少24.44%—80.33%; 第5天, 处理组T6E2、T4E4、T8E0、T0E8和T2E6r值比对照组分别降低30.00%、37.14%、41.43%、60.00% 和65.71%。研究结果显示睾丸酮和雌二醇对卜氏晶囊轮虫生长发育及种群增长率有明显影响, 并且卜氏晶囊轮虫对雌二醇表现更为敏感, 2种激素呈现出协同作用。

     

    Abstract: The effects of testosterone, estradiol and the combination of the two hormones on the main development stages and population growth rate of Asplanchna brightwelli were studied with ecological experiment methods. The results showed that the 48h LC50 of testosterone (T) was 9.56 mg/L, Y=1.9793X+3.0591 (R2=0.9211); 48h LC50 of estradiol (E) was 8.18 mg/L, Y=3.5292X+1.7788 (R2=0.9195). Testosterone and estradiol significantly reduced the lifespan, the number of offspring, and population growth rate (r) except the condition under the concentration of 0.5 mg/L. Treatment with testosterone at the concentration of 8 mg/L significantly prolonged the pre-reproductive time by 11.71%, and decreased the lifespan and the number of offspring by 52.22% and 82.20%, respectively. Treatment with estradiol at the concentration of 8 mg/L significantly prolonged the pre-reproductive time by 7.10%, and decreased the lifespan and the number of offspring by 49.75% and 83.33%, respectively. When the two hormones were at a total concentration of 8 mg/L, compared with the control groups, the pre-reproductive period significantly prolonged by 34.17%—48.01%; the mean life significantly shortened by 31.56%—42.12%; the number of offspring reduced by 24.44%—80.33%. At the 5d, the population growth rate of rotifers significantly declined by 30.00%, 37.14%, 41.43%, 60.00% and 65.71% when they were exposed to T6E2, T4E4, T8E0, T0E8 and T2E6, respectively. Consequently, testosterone and estradiol showed significant effects on the durations of different development stages of Asplanchna brightwelli and the characteristics of its population growth. Simultaneously, the two hormones showed synergistic effects and the rotifers were more sensitive to estradiol.

     

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