Abstract: The information on Rotifera of Hainan Island is limited. To examine the species composition of the Rotifera on Hainan Island, 134 samples were collected from 134 freshwater habits (reservoirs, ponds, temporal ponds, brooks, rivers and rice fields) in November 2010, May and August 2011. Twenty-four species of the genera Trichocerca were identified. Trichocerca agnatha, T. obtusidens, T. rosea and T. pygocera are new to the Oriental Zone, and T. agnatha and T. braziliensis are new to China. Trichocerca pusilla, T. similis, T. braziliensis and T. tenuior are the most common species in this genus. The majority of Trichocerca on Hainan island was widely distributed. There are seventeen cosmopolitan species, three cold-water taxa and two warm-water taxa. The diversity of Trichocerca on Hainan island is much higher than that in the other regions of China, and Bray-Curtis dissimilarity of Trichocerca between Hainan island and the other regions of China increases with higher latitudes.