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任诗思, 齐志涛, 昌鸣先. 大鲵短型肽聚糖识别蛋白PGRP-S的克隆及其功能研究[J]. 水生生物学报, 2018, 42(3): 473-479. DOI: 10.7541/2018.059
引用本文: 任诗思, 齐志涛, 昌鸣先. 大鲵短型肽聚糖识别蛋白PGRP-S的克隆及其功能研究[J]. 水生生物学报, 2018, 42(3): 473-479. DOI: 10.7541/2018.059
REN Shi-Si, QI Zhi-Tao, CHANG Ming-Xian. CLONING AND FUNCTIONAL STUDY OF PGRP-S IN CHINESE GIANT SALAMANDER ANDRIAS DAVIDIANUS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(3): 473-479. DOI: 10.7541/2018.059
Citation: REN Shi-Si, QI Zhi-Tao, CHANG Ming-Xian. CLONING AND FUNCTIONAL STUDY OF PGRP-S IN CHINESE GIANT SALAMANDER ANDRIAS DAVIDIANUS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(3): 473-479. DOI: 10.7541/2018.059

大鲵短型肽聚糖识别蛋白PGRP-S的克隆及其功能研究

CLONING AND FUNCTIONAL STUDY OF PGRP-S IN CHINESE GIANT SALAMANDER ANDRIAS DAVIDIANUS

  • 摘要: 实验构建了大鲵短型肽聚糖识别蛋白PGRP-S的真核表达质粒, 并对其功能进行了初步的研究。序列分析显示, 所克隆的大鲵PGRP-S的N端不含有信号肽; 其编码的氨基酸序列具有2个相距较近的半胱氨酸残基以及2个Zn2+结合位点。Western-blotting检测结果显示大鲵PGRP-S既可分泌到胞外, 也可滞留在胞内。体外抗菌实验的结果表明, 过表达大鲵PGRP-S能显著抑制迟缓爱德华氏菌(Edwardsiella tarda)在HEK293T细胞内和胞外培养基中的增殖。此外, 过表达大鲵PGRP-S能诱导NF-κB启动子的活性; 能结合Lys-type和Dap-type的肽聚糖但不能降解它们。研究结果表明大鲵PGRP-S在功能上类似于哺乳动物而有别于硬骨鱼类短型的肽聚糖识别蛋白。

     

    Abstract: Peptidoglycan recognition proteins (PGRPs) are a class of pattern recognition receptors that are highly conserved from invertebrates to mammals. So far, PGRP is rarely reported in vertebrate species except for bony fish and mammals. This study constructed the eukaryotic expression plasmid of PGRP-S, a short peptidoglycan recognition protein in Chinese giant salamander, and explored its functins. Sequence analysis showed that the N-terminal of PGRP-S had no signal peptide, but had two closely spaced cysteine residues and two Zn2+ binding sites. Moreover, PGRP-S could be secreted to the exterior and also be retained within the cells. In vitro antibacterial experiments revealed that overexpression of PGRP-S significantly inhibited the proliferation of Edwardsiella tarda in HEK293T cells intracellularly and extracellularly. In addition, overexpression of PGRP-S enhanced NF-κB promoter activity and bound Lys-type and Dap-type peptidoglycan but without degradation ability.These results indicate that the Chinese giant salamander PGRP-S is functionally similar to mammalian PGRP-S.

     

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