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戢水玲, 高宏. 集胞藻PCC 6803高产精氨酸藻株的紫外诱变选育[J]. 水生生物学报, 2018, 42(3): 635-640. DOI: 10.7541/2018.079
引用本文: 戢水玲, 高宏. 集胞藻PCC 6803高产精氨酸藻株的紫外诱变选育[J]. 水生生物学报, 2018, 42(3): 635-640. DOI: 10.7541/2018.079
JI Shui-Ling, GAO Hong. CREATION OF HIGH ARGININE PRODUCING STRAIN OF SYNECHOCYSTIS SP. PCC 6803 BY UV MUTAGENESIS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(3): 635-640. DOI: 10.7541/2018.079
Citation: JI Shui-Ling, GAO Hong. CREATION OF HIGH ARGININE PRODUCING STRAIN OF SYNECHOCYSTIS SP. PCC 6803 BY UV MUTAGENESIS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(3): 635-640. DOI: 10.7541/2018.079

集胞藻PCC 6803高产精氨酸藻株的紫外诱变选育

CREATION OF HIGH ARGININE PRODUCING STRAIN OF SYNECHOCYSTIS SP. PCC 6803 BY UV MUTAGENESIS

  • 摘要: 精氨酸在医药和食品工业上具有广泛用途。集胞藻PCC 6803是单细胞蓝藻, 能利用工业废气(主要成分是氮氧化物NOx)与水反应生成的硝酸盐和亚硝酸盐合成氨基酸等化合物, 因而选育高产精氨酸藻株, 不仅能提高精氨酸产量, 而且能去除工业废气中的NOx, 具有潜在的应用前景。研究在集胞藻PCC 6803中利用紫外诱变, 筛选抗0.8 g/L D-精氨酸和抗0.2 g/L 6-氮尿嘧啶的突变株, 选育到了一株精氨酸产量显著提高的突变株#13807-111-55, 它每OD730值细胞的胞外精氨酸产量相比出发株提高了62.3倍, 达到(0.76±0.1) mg/(L·OD730), 总精氨酸产量相比出发株提高了6.0倍, 达到(0.82±0.08) mg/(L·OD730)。该突变株每OD730值细胞的胞外精氨酸产量明显高于胞内, 表明该突变藻株是精氨酸分泌型, 因而具有潜在的应用前景。

     

    Abstract: Arginine has been widely used in medicine and food industry. Synechocystis sp. PCC 6803, a unicellular cyanobacterium, synthesize amino acids and other compounds using nitrate or nitrite as the nitrogen source. In flue gases released by industrial production, NOx (nitrogen oxides) can react with water to form nitrate and nitrite, which can be utilized by cyanobacteria. Hence, creating high arginine producing strain of Synechocystis sp. PCC 6803 has a strong application prospects, in denitrification of industrial flue gases. This study reported the successful creation of a high arginine producing strain #13807-111-55 of Synechocystis sp. PCC 6803 by 0.8 g/L D-arginine and 0.2 g/L 6-azaruacil resistant mutants with UV mutagenesis. Compared with the parent strain #13807, extracellular arginine production per OD730 of #13807-111-55 increased 62.3-fold, reaching (0.76±0.1) mg/(L·OD730). Total arginine production per OD730 of #13807-111-55 increased about 6.0-fold, reaching (0.82±0.08) mg/(L·OD730). Extracellular arginine production per OD730 of the mutant was significantly higher than that of intracellular, showing that the strain was the arginine-secreting mutant with strong production potential.

     

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