周期性饥饿再投喂对长蛸存活、生长以及肌肉脂肪酸和氨基酸的影响

EFFECTS OF CYCLICAL STARVATION-REFEEDING ON SURVIVAL, GROWTH, AND MUSCLE FATTY ACIDS, AMINO ACIDS OF OCTOPUS MINOR

  • 摘要: 在19.8—22.2℃条件下, 采用周期性饥饿再投喂的方法, 研究不同投喂模式对长蛸Octopus minor, 初始体重(94.29±9.35) g, 初始胴背长(53.25±5.25) mm的存活、生长以及脂肪酸和氨基酸的影响。实验分为4个组包括对照组(持续投喂)、S1F5组(周期性饥饿1d再投喂5d)、S2F4组(周期性饥饿2d再投喂4d)和S3F3组(周期性饥饿3d再投喂3d), 持续24d。实验结果如下: 随饥饿时间的延长, 增重率、肝体比、体重变化量以及终末体重四个指标均呈现出先上升后下降的趋势, 其中S1F5组的值均显著高于对照组, 而S3F3组的值除肝体比外均显著低于对照组。长蛸的成活率随饥饿时间的增长呈现出下降趋势, 但各实验组均与对照组差异不显著; 摄食量随饥饿时间的延长, 表现出上升趋势, 且三个实验组的值均显著高于对照组。在长蛸肌肉脂肪酸中, 饱和脂肪酸(SFA)、不饱和脂肪酸(UFA)、单不饱和脂肪酸(MUFA)以及多不饱和脂肪酸(PUFA)的含量均与对照组差异不显著, 但S1F5组的值与对照组相比出现了一定程度的升高; 在氨基酸含量上, 各实验组的必需氨基酸总量(TEAA)、氨基酸总量(TAA)以及必需氨基酸总量/氨基酸总量(TEAA/TAA)的值均与对照组差异不显著。综上所述, S1F5组和S2F4组长蛸出现了补偿生长现象, 且S1F5组长蛸具备超补偿生长能力。因此, 在长蛸的人工养殖过程中, 为保证其养殖效果, 建议采用周期性饥饿1d再投喂5d的投喂模式。

     

    Abstract: This study investigated effects of different cyclical starvation-refeeding modes on survival, growth, and fatty acids and amino acids of Octopus minor with initial weight of (94.29±9.35) g and initial mantle length of (53.25±5.25) mm under the seawater temperature of 19.8—22.2℃ in four groups including control group (continuous feeding), S1F5 group (cyclic starvation 1d and refeeding 5d), S2F4 group (cyclic starvation 2d and refeeding 4d), S3F3 group (cyclic starvation 3d and refeeding 3d) and for 24d. The results revealed that growth rate, hepatosomatic index, weight gain ratio and final weight increased at first and then decreased with the increased starvation time, in which all the indicators of S1F5 group were significantly higher than those of the control group, but all indicators except the hepatosomatic index of S3F3 group were significantly lower than those of the control group. The survival rate of O. minor decreased with enhanced starvation time but without significant differences among all groups. The food ingestion enhanced with the increased starvation phase, and their values of all three experimental groups were significantly higher than the control group. The content of SFA, UFA, MUFA and PUFA in fatty acids was not significantly different with the control group with the highest values in S1F5. The values of TEAA, TAA and TEAA/TAA of O. minor were not significantly different from the control group. In conclusion, O. minor from S1F5 and S2F4 groups exhibited compensate growth and S1F5 group showed over-compensatory growth. Therefore, the feeding mode of cyclical 1d starvation and 5d re-feeding can be used in O. minor culture.

     

/

返回文章
返回